Chan Woo Song, Dong In Kim, Sol Choi, Jae Won Jang & Sang Yup Lee (2013). Metabolic engineering of Escherichia coli for the production of fumaric acid. Biotechnology & Bioengineering.

Strains

• Escherichia coli CWF0
• Escherichia coli CWF1
• Escherichia coli CWF2
• Escherichia coli CWF3
• Escherichia coli CWF4
• Escherichia coli CWF41
• Escherichia coli CWF51
• Escherichia coli CWF61
• Escherichia coli CWF71
• Escherichia coli CWF81
• Escherichia coli CWF611
• Escherichia coli CWF811
• Escherichia coli CWF812
• Escherichia coli W3110

Plasmids

• pTac15kppc
• pTac15K

Metabolite production

• acetate
  Titer
  • 1.65 g/L (Escherichia coli CWF61 in Flask)
  • 2.29 g/L (Escherichia coli CWF51 in Flask)
  • 1.13 g/L (Escherichia coli CWF81 in Flask)
  • 2.0 g/L (Escherichia coli CWF811 in Batch)
  • 2.0 g/L (Escherichia coli CWF812 in Batch)
  • 9.55 g/L (Escherichia coli CWF811 in Fed-Batch)
  • 5.24 g/L (Escherichia coli CWF812 in Fed-Batch)
  Substrate yield
  • 0.11 g/g of sustrate (Escherichia coli CWF61 in Flask)
  • 0.153 g/g of sustrate (Escherichia coli CWF51 in Flask)
  • 0.075 g/g of sustrate (Escherichia coli CWF81 in Flask)
  • 0.1 g/g of sustrate (Escherichia coli CWF812 in Batch)
  • 0.1 g/g of sustrate (Escherichia coli CWF811 in Batch)
  • 0.132 g/g of sustrate (Escherichia coli CWF811 in Fed-Batch)
  • 0.072 g/g of sustrate (Escherichia coli CWF812 in Fed-Batch)
• fumarate
  Titer
  • 1.45 g/L (Escherichia coli CWF4 in Flask)
  • 4.09 g/L (Escherichia coli CWF41 in Flask)
  • 4.9 g/L (Escherichia coli CWF51 in Flask)
  • 5.34 g/L (Escherichia coli CWF61 in Flask)
  • 5.85 g/L (Escherichia coli CWF81 in Flask)
  • 6.25 g/L (Escherichia coli CWF811 in Flask)
  • 8.23 g/L (Escherichia coli CWF811 in Batch)
  • 26.2 g/L (Escherichia coli CWF811 in Fed-Batch)
  • 7.85 g/L (Escherichia coli CWF812 in Batch)
  • 28.2 g/L (Escherichia coli CWF812 in Fed-Batch)
  • 5.06 g/L (Escherichia coli CWF611 in Flask)
  Substrate yield
  • 0.097 g/g of sustrate (Escherichia coli CWF4 in Flask)
  • 0.273 g/g of sustrate (Escherichia coli CWF41 in Flask)
  • 0.327 g/g of sustrate (Escherichia coli CWF51 in Flask)
  • 0.35 g/g of sustrate (Escherichia coli CWF61 in Flask)
  • 0.41 g/g of sustrate (Escherichia coli CWF81 in Flask)
  • 0.417 g/g of sustrate (Escherichia coli CWF811 in Flask)
  • 0.415 g/g of sustrate (Escherichia coli CWF811 in Batch)
  • 0.361 g/g of sustrate (Escherichia coli CWF811 in Fed-Batch)
  • 0.394 g/g of sustrate (Escherichia coli CWF812 in Batch)
  • 0.389 g/g of sustrate (Escherichia coli CWF812 in Fed-Batch)
  • 0.343 g/g of sustrate (Escherichia coli CWF611 in Flask)
  Volumetric productivity
  • 0.305 g/L/h (Escherichia coli CWF811 in Batch)
  • 0.252 g/L/h (Escherichia coli CWF811 in Fed-Batch)
  • 0.393 g/L/h (Escherichia coli CWF812 in Batch)
  • 0.448 g/L/h (Escherichia coli CWF812 in Fed-Batch)
  Specific production rate
  • 0.201 g/g DCW/h (Escherichia coli CWF811 in Batch)
  • 0.238 g/g DCW/h (Escherichia coli CWF812 in Batch)