Dong-Eun Chang, Heung-Chae Jung, Joon-Shick Rhee & Jae-Gu Pan (1999). Homofermentative Production of d- orl-Lactate in Metabolically Engineered Escherichia coli RR1. Applied and Environmental Microbiology.

Strains

• Escherichia coli RR1
• Escherichia coli JP201
• Escherichia coli JP202
• Escherichia coli JP203
• Escherichia coli JP204
• Escherichia coli JP205
• Escherichia coli JP204(pLS65)
• Escherichia coli JP205(pLS65)
• Escherichia coli W3110
• Escherichia coli BL21(DE3)

Plasmids

• pLS65
• pUC19

Metabolite production

• acetate
  Substrate yield
  • 0.6 mmol/mmol of sustrate (Escherichia coli RR1 in Flask)
  • 0.06 mmol/mmol of sustrate (Escherichia coli JP201 in Flask)
  • 0.12 mmol/mmol of sustrate (Escherichia coli JP204 in Flask)
  • 0.04 mmol/mmol of sustrate (Escherichia coli JP204(pLS65) in Flask)
• ethanol
  Substrate yield
  • 0.58 mmol/mmol of sustrate (Escherichia coli RR1 in Flask)
  • 0.07 mmol/mmol of sustrate (Escherichia coli JP201 in Flask)
  • 0.42 mmol/mmol of sustrate (Escherichia coli JP204 in Flask)
  • 0.24 mmol/mmol of sustrate (Escherichia coli JP204(pLS65) in Flask)
• succinate
  Substrate yield
  • 0.13 mmol/mmol of sustrate (Escherichia coli RR1 in Flask)
  • 0.19 mmol/mmol of sustrate (Escherichia coli JP201 in Flask)
  • 0.25 mmol/mmol of sustrate (Escherichia coli JP204 in Flask)
  • 0.2 mmol/mmol of sustrate (Escherichia coli JP204(pLS65) in Flask)
  Titer
  • 0.0 g/L (Escherichia coli JP203 in Fed-Batch)
  • 9.0 g/L (Escherichia coli JP201 in Fed-Batch)
  • 5.4 g/L (Escherichia coli JP204(pLS65) in Fed-Batch)
• pyruvate
  Substrate yield
  • 0.03 mmol/mmol of sustrate (Escherichia coli RR1 in Flask)
  • 0.02 mmol/mmol of sustrate (Escherichia coli JP201 in Flask)
  • 0.36 mmol/mmol of sustrate (Escherichia coli JP204 in Flask)
  • 0.0 mmol/mmol of sustrate (Escherichia coli JP204(pLS65) in Flask)
• formate
  Substrate yield
  • 1.13 mmol/mmol of sustrate (Escherichia coli RR1 in Flask)
  • 0.08 mmol/mmol of sustrate (Escherichia coli JP201 in Flask)
  • 0.44 mmol/mmol of sustrate (Escherichia coli JP204 in Flask)
  • 0.21 mmol/mmol of sustrate (Escherichia coli JP204(pLS65) in Flask)
• (R)-lactate
  Substrate yield
  • 0.26 mmol/mmol of sustrate (Escherichia coli RR1 in Flask)
  • 1.52 mmol/mmol of sustrate (Escherichia coli JP201 in Flask)
  • 0.9 g/g of sustrate (Escherichia coli JP203 in Fed-Batch)
  Titer
  • 47.0 g/L (Escherichia coli JP201 in Fed-Batch)
  • 60.0 g/L (Escherichia coli JP201 in Fed-Batch)
  • 62.2 g/L (Escherichia coli JP203 in Fed-Batch)
  Volumetric productivity
  • 1.09 g/L/h (Escherichia coli JP201 in Fed-Batch)
  • 1.04 g/L/h (Escherichia coli JP203 in Fed-Batch)
• (S)-lactate
  Substrate yield
  • 0.72 mmol/mmol of sustrate (Escherichia coli JP204(pLS65) in Flask)
  • 0.69 g/g of sustrate (Escherichia coli JP204(pLS65) in Fed-Batch)
  • 0.0 mmol/mmol of sustrate (Escherichia coli JP204 in Flask)
  Titer
  • 4.18 g/L (Escherichia coli JP205(pLS65) in Fed-Batch)
  • 45.0 g/L (Escherichia coli JP204(pLS65) in Fed-Batch)
  Volumetric productivity
  • 0.146 g/L/h (Escherichia coli JP205(pLS65) in Fed-Batch)