Yu Deng, Ning Ma, Kangjia Zhu, Yin Mao, Xuetuan Wei & Yunying Zhao (2018). Balancing the carbon flux distributions between the TCA cycle and glyoxylate shunt to produce glycolate at high yield and titer in Escherichia coli. Metabolic Engineering.
Strains
- Escherichia coli MG1655(DE3)
- Escherichia coli Mgly2
- Escherichia coli Mgly3
- Escherichia coli Mgly4
- Escherichia coli Mgly5
- Escherichia coli Mgly11
- Escherichia coli Mgly12
- Escherichia coli Mgly13
- Escherichia coli Mgly12P
- Escherichia coli Mgly134
- Escherichia coli Mgly334
- Escherichia coli Mgly1
- Escherichia coli Mgly434
- Escherichia coli Mgly534
- Escherichia coli EYX-2
- Escherichia coli EYX-3
- Escherichia coli MG1655
Plasmids
Metabolite production
-
acetate
-
Titer
- 10.63 g/L (Escherichia coli Mgly434 in Fed-Batch)
-
glycolate
-
Titer
- 1.8 g/L (Escherichia coli Mgly534 in Flask)
- 2.822 g/L (Escherichia coli Mgly434 in Flask)
- 65.5 g/L (Escherichia coli Mgly434 in Fed-Batch)
- 2.4 g/L (Escherichia coli Mgly334 in Flask)
- 1.1 g/L (Escherichia coli Mgly11 in Flask)
- 0.95 g/L (Escherichia coli Mgly12 in Flask)
- 1.13 g/L (Escherichia coli Mgly13 in Flask)
- 1.96 g/L (Escherichia coli Mgly12P in Flask)
- 1.37 g/L (Escherichia coli Mgly134 in Flask) Substrate yield
- 0.679 g/g of sustrate (Escherichia coli Mgly534 in Flask)
- 0.79 g/g of sustrate (Escherichia coli Mgly434 in Flask)
- 0.765 g/g of sustrate (Escherichia coli Mgly434 in Fed-Batch)
- 0.75 g/g of sustrate (Escherichia coli Mgly334 in Flask)
- 0.2 g/g of sustrate (Escherichia coli Mgly11 in Flask)
- 0.189 g/g of sustrate (Escherichia coli Mgly12 in Flask)
- 0.385 g/g of sustrate (Escherichia coli Mgly13 in Fed-Batch)
- 0.385 g/g of sustrate (Escherichia coli Mgly13 in Flask)
- 0.287 g/g of sustrate (Escherichia coli Mgly12P in Flask)
- 0.504 g/g of sustrate (Escherichia coli Mgly134 in Fed-Batch)
- 0.534 g/g of sustrate (Escherichia coli Mgly134 in Flask)
- 0.48 g/g of sustrate (Escherichia coli EYX-2 in Flask)
- 0.57 g/g of sustrate (Escherichia coli EYX-3 in Flask)