Xiaoxiang Dong, Xiulai Chen, Yuanyuan Qian, Yuancai Wang, Li Wang, Weihua Qiao & Liming Liu (2016). Metabolic engineering of Escherichia coli W3110 to produce L‐malate. Biotechnology & Bioengineering.

Strains

• Escherichia coli F0201
• Escherichia coli F0301
• Escherichia coli F0401
• Escherichia coli F0501
• Escherichia coli F0601
• Escherichia coli F0511
• Escherichia coli F0701
• Escherichia coli F0801
• Escherichia coli F0901
• Escherichia coli F0711
• Escherichia coli F0811
• Escherichia coli F0911
• Escherichia coli F0921
• Escherichia coli F0931
• Escherichia coli W3110

Plasmids

• pTrcHisA
• pTrcHisA-ME₂
• pTrcHisA-ME₄
• pTrcHisA-C490S
• pTrcHisA-C490S-pos5
• pTrcHisA-C490S-ldhA

Metabolite production

• (R)-lactate
  Titer
  • 6.34 g/L (Escherichia coli W3110 in Flask)
  • 0.0 g/L (Escherichia coli F0201 in Flask)
  • 0.0 g/L (Escherichia coli F0401 in Flask)
  • 0.0 g/L (Escherichia coli F0501 in Flask)
  • 0.0 g/L (Escherichia coli F0601 in Flask)
  • 0.0 g/L (Escherichia coli F0301 in Flask)
• acetate
  Titer
  • 4.72 g/L (Escherichia coli W3110 in Flask)
  • 5.48 g/L (Escherichia coli F0201 in Flask)
  • 3.71 g/L (Escherichia coli F0301 in Flask)
  • 3.48 g/L (Escherichia coli F0401 in Flask)
  • 0.22 g/L (Escherichia coli F0501 in Flask)
  • 0.39 g/L (Escherichia coli F0601 in Flask)
• formate
  Titer
  • 2.34 g/L (Escherichia coli W3110 in Flask)
  • 2.25 g/L (Escherichia coli F0201 in Flask)
  • 2.64 g/L (Escherichia coli F0301 in Flask)
  • 0.56 g/L (Escherichia coli F0401 in Flask)
  • 0.52 g/L (Escherichia coli F0501 in Flask)
  • 0.52 g/L (Escherichia coli F0601 in Flask)
• ethanol
  Titer
  • 1.43 g/L (Escherichia coli W3110 in Flask)
  • 1.67 g/L (Escherichia coli F0201 in Flask)
  • 1.23 g/L (Escherichia coli F0301 in Flask)
  • 1.25 g/L (Escherichia coli F0401 in Flask)
  • 2.54 g/L (Escherichia coli F0501 in Flask)
  • 0.0 g/L (Escherichia coli F0601 in Flask)
• pyruvate
  Titer
  • 12.35 g/L (Escherichia coli F0201 in Flask)
  • 15.16 g/L (Escherichia coli F0301 in Flask)
  • 15.3 g/L (Escherichia coli F0401 in Flask)
  • 20.9 g/L (Escherichia coli F0501 in Flask)
  • 16.08 g/L (Escherichia coli F0601 in Flask)
  • 8.61 g/L (Escherichia coli F0501 in Flask)
  • 7.4 g/L (Escherichia coli F0511 in Flask)
  • 16.54 g/L (Escherichia coli F0931 in Flask)
  • 5.54 g/L (Escherichia coli W3110 in Flask)
• citrate
  Titer
  • 2.85 g/L (Escherichia coli F0501 in Flask)
  • 2.92 g/L (Escherichia coli F0511 in Flask)
• succinate
  Titer
  • 4.08 g/L (Escherichia coli F0501 in Flask)
  • 5.39 g/L (Escherichia coli F0511 in Flask)
  • 0.98 g/L (Escherichia coli F0931 in Flask)
• fumarate
  Titer
  • 0.19 g/L (Escherichia coli F0931 in Flask)
• (S)-malate
  Biomass-specific yield
  • 0.19 g/g DCW (Escherichia coli F0501 in Flask)
  • 0.41 g/g DCW (Escherichia coli F0511 in Flask)
  Titer
  • 1.97 g/L (Escherichia coli F0501 in Flask)
  • 3.62 g/L (Escherichia coli F0511 in Flask)
  • 7.78 g/L (Escherichia coli F0911 in Flask)
  • 21.65 g/L (Escherichia coli F0931 in Flask)
  • 6.87 g/L (Escherichia coli F0811 in Flask)
  • 5.63 g/L (Escherichia coli F0711 in Flask)
  Volumetric productivity
  • 0.11 g/L/h (Escherichia coli F0911 in Flask)
  • 0.3 g/L/h (Escherichia coli F0931 in Flask)
  Substrate yield
  • 0.36 g/g of sustrate (Escherichia coli F0931 in Flask)