Xianzhong Chen, Mingming Li, Li Zhou, Wei Shen, Govender Algasan, You Fan & Zhengxiang Wang (2014). Metabolic engineering of Escherichia coli for improving shikimate synthesis from glucose. Bioresource Technology.

Strains

• Escherichia coli SA1
• Escherichia coli SA2
• Escherichia coli SA3
• Escherichia coli SA4
• Escherichia coli SA4*
• Escherichia coli SA5
• Escherichia coli B0013
• Escherichia coli SA5/pTH-aroG*
• Escherichia coli SA5/pTH-aroG*-ppsA
• Escherichia coli SA5/pTH-aroG*-ppsA-tktA

Plasmids

• pTH-aroG^fbr
• pTH-aroG^fbr-ppsA
• pTH-aroG^fbr-ppsA-tktA
• pTH18kr

Metabolite production

• acetate
  Titer
  • 5.89 g/L (Escherichia coli W3110 in Flask)
  • 6.45 g/L (Escherichia coli B0013 in Flask)
  • 6.18 g/L (Escherichia coli SA1 in Flask)
  • 5.67 g/L (Escherichia coli SA2 in Flask)
  • 3.06 g/L (Escherichia coli SA3 in Flask)
  • 2.67 g/L (Escherichia coli SA4* in Flask)
  • 2.68 g/L (Escherichia coli SA4 in Flask)
  • 1.15 g/L (Escherichia coli SA5 in Flask)
• L-quinate
  Titer
  • 0.0 mg/L (Escherichia coli B0013 in Flask)
  • 0.0 mg/L (Escherichia coli W3110 in Flask)
  • 0.0 mg/L (Escherichia coli SA1 in Flask)
  • 45.45 mg/L (Escherichia coli SA2 in Flask)
  • 128.17 mg/L (Escherichia coli SA3 in Flask)
  • 89.23 mg/L (Escherichia coli SA4* in Flask)
  • 37.62 mg/L (Escherichia coli SA4 in Flask)
  • 40.53 mg/L (Escherichia coli SA5 in Flask)
  • 0.68 g/L (Escherichia coli SA5/pTH-aroG*-ppsA-tktA in Fed-Batch)
  Substrate yield
  • 0.0136 g/g of sustrate (Escherichia coli SA5/pTH-aroG*-ppsA-tktA in Fed-Batch)
  • 0.0059 g/g of sustrate (Escherichia coli SA5/pTH-aroG*-ppsA-tktA in Flask)
• 3-dehydroquinate
  Titer
  • 0.87 g/L (Escherichia coli SA5/pTH-aroG*-ppsA-tktA in Fed-Batch)
  Substrate yield
  • 0.0174 g/g of sustrate (Escherichia coli SA5/pTH-aroG*-ppsA-tktA in Fed-Batch)
  • 0.0288 g/g of sustrate (Escherichia coli SA5/pTH-aroG*-ppsA-tktA in Flask)
• shikimate
  Titer
  • 1.13 mg/L (Escherichia coli W3110 in Flask)
  • 1.63 mg/L (Escherichia coli B0013 in Flask)
  • 21.45 mg/L (Escherichia coli SA1 in Flask)
  • 67.81 mg/L (Escherichia coli SA2 in Flask)
  • 417.2 mg/L (Escherichia coli SA3 in Flask)
  • 576.17 mg/L (Escherichia coli SA4 in Flask)
  • 670.04 mg/L (Escherichia coli SA5 in Flask)
  • 900.0 mg/L (Escherichia coli SA5/pTH-aroG* in Flask)
  • 1080.0 mg/L (Escherichia coli SA5/pTH-aroG*-ppsA in Flask)
  • 1207.0 mg/L (Escherichia coli SA5/pTH-aroG*-ppsA-tktA in Flask)
  • 14.6 g/L (Escherichia coli SA5/pTH-aroG*-ppsA-tktA in Fed-Batch)
  • 385.62 mg/L (Escherichia coli SA4* in Flask)
  Substrate yield
  • 85.1 mg/g of sustrate (Escherichia coli SA5/pTH-aroG*-ppsA-tktA in Flask)
  • 0.2925 g/g of sustrate (Escherichia coli SA5/pTH-aroG*-ppsA-tktA in Fed-Batch)
  Volumetric productivity
  • 0.047 g/L/h (Escherichia coli SA5/pTH-aroG*-ppsA-tktA in Flask)
  • 0.26 g/L/h (Escherichia coli SA5/pTH-aroG*-ppsA-tktA in Fed-Batch)