L Stols & M I Donnelly (1997). Production of succinic acid through overexpression of NAD(+)-dependent malic enzyme in an Escherichia coli mutant. Applied and Environmental Microbiology.

Strains

• Escherichia coli W1485
• Escherichia coli LS1
• Escherichia coli FMJ123
• Escherichia coli NZN111
• Escherichia coli NZN111 (pMEE1)
• Escherichia coli LS1 (pMEE1)
• Escherichia coli FMJ123 (pMEE1)

Plasmids

• pMEE1
• pTrc99A

Metabolite production

• (R)-lactate
  Titer
  • 0.19 g/L (Escherichia coli LS1 (pMEE1) in Flask)
  • 14.22 g/L (Escherichia coli FMJ123 (pMEE1) in Flask)
  • 0.0 g/L (Escherichia coli NZN111 (pMEE1) in Flask)
• formate
  Titer
  • 4.36 g/L (Escherichia coli LS1 (pMEE1) in Flask)
  • 0.0 g/L (Escherichia coli FMJ123 (pMEE1) in Flask)
  • 0.0 g/L (Escherichia coli NZN111 (pMEE1) in Flask)
• acetate
  Titer
  • 4.75 g/L (Escherichia coli LS1 (pMEE1) in Flask)
  • 0.42 g/L (Escherichia coli FMJ123 (pMEE1) in Flask)
  • 0.9 g/L (Escherichia coli NZN111 (pMEE1) in Flask)
• ethanol
  Titer
  • 4.37 g/L (Escherichia coli LS1 (pMEE1) in Flask)
  • 0.2 g/L (Escherichia coli FMJ123 (pMEE1) in Flask)
  • 0.7 g/L (Escherichia coli NZN111 (pMEE1) in Flask)
• succinate
  Titer
  • 2.3 g/L (Escherichia coli LS1 (pMEE1) in Flask)
  • 12.8 g/L (Escherichia coli NZN111 (pMEE1) in Flask)
  • 1.38 g/L (Escherichia coli FMJ123 (pMEE1) in Flask)