Ming-De Deng, David K. Severson, Alan D. Grund, Sarah L. Wassink, Richard P. Burlingame, Alan Berry, Jeffrey A. Running, Candice A. Kunesh, Linsheng Song, Thomas A. Jerrell & Reinhardt A. Rosson (2005). Metabolic engineering of Escherichia coli for industrial production of glucosamine and N-acetylglucosamine. Metabolic Engineering.

Strains

• Escherichia coli ATCC 25947
• Escherichia coli 7101-17 (DE3)
• Escherichia coli 2123-12
• Escherichia coli 2123-54
• Escherichia coli 7107-18
• Escherichia coli 7107-88
• Escherichia coli 7107-87
• Escherichia coli 7107-93
• Escherichia coli 7107-117
• Escherichia coli 7107-92
• Escherichia coli 7107-607
• Escherichia coli 7107-608
• Escherichia coli 7107-609
• Escherichia coli 7107-612

Plasmids

• pET24d(+)
• pET24d(+)/T7lac-AtGNA1
• pET24d(+)/T7lac-ScGNA1
• pET24d(+)/T7lac-CaGNA1

Metabolite production

• acetate
  Titer
  • 4.4 g/L (Escherichia coli 7107-88 in Flask)
  • 0.0 g/L (Escherichia coli 7107-87 in Flask)
  • 0.5 g/L (Escherichia coli 7107-117 in Flask)
  • 0.0 g/L (Escherichia coli 7107-93 in Flask)
• D-glucosamine
  Titer
  • 4.0 mg/L (Escherichia coli ATCC 25947 in Flask)
  • 75.0 mg/L (Escherichia coli 2123-12 in Flask)
  • 6.0 g/L (Escherichia coli 2123-54 in Flask)
  • 17.0 g/L (Escherichia coli 7107-18 in Fed-Batch)
• N-acetyl-β-D-glucosamine
  Titer
  • 0.0 g/L (Escherichia coli 7107-88 in Flask)
  • 11.7 g/L (Escherichia coli 7107-87 in Flask)
  • 22.4 g/L (Escherichia coli 7107-87 in Flask)
  • 50.0 g/L (Escherichia coli 7107-87 in Fed-Batch)
  • 8.0 g/L (Escherichia coli 7107-93 in Flask)
  • 5.1 g/L (Escherichia coli 7107-117 in Flask)
  • 110.0 g/L (Escherichia coli 7107-607 in Fed-Batch)