Jin Hwan Park, Jae Eun Oh, Kwang Ho Lee, Ji Young Kim & Sang Yup Lee (2012). Rational Design of Escherichia coli for l‑Isoleucine Production. ACS Synthetic Biology.

Strains

• Escherichia coli WL3110
• Escherichia coli TH20
• Escherichia coli ILE01
• Escherichia coli ILE02
• Escherichia coli ILE03
• Escherichia coli WygaZH
• Escherichia coli Top10
• Escherichia coli ILE01 (pTacilvAIH, pBRthrABC)
• Escherichia coli WL (pTac15K)
• Escherichia coli WL (pTacilvIH)
• Escherichia coli ILE01 (pTacilvAIH, pBRthrABCygaZH)
• Escherichia coli ILE02 (pTacilvAIH, pBRthrABCygaZH)
• Escherichia coli ILE03 (pTacilvAIH, pBRthrABCygaZH)
• Escherichia coli W3110

Plasmids

• pTac15K
• pTacilvIH
• pTacilvAIH
• pBRThrABC
• pBRthrABCygaZH
• pTrc184ygaZH

Metabolite production

• L-isoleucine
  Titer
  • 0.322 g/L (Escherichia coli ILE01 (pTacilvAIH, pBRthrABC) in Flask)
  • 1.11 g/L (Escherichia coli ILE01 (pTacilvAIH, pBRthrABCygaZH) in Flask)
  • 2.11 g/L (Escherichia coli ILE02 (pTacilvAIH, pBRthrABCygaZH) in Flask)
  • 2.83 g/L (Escherichia coli ILE03 (pTacilvAIH, pBRthrABCygaZH) in Flask)
  • 8.72 g/L (Escherichia coli ILE03 (pTacilvAIH, pBRthrABCygaZH) in Fed-Batch)
  • 9.46 g/L (Escherichia coli ILE03 (pTacilvAIH, pBRthrABCygaZH) in Fed-Batch)
  Volumetric productivity
  • 0.121 g/L/h (Escherichia coli ILE03 (pTacilvAIH, pBRthrABCygaZH) in Fed-Batch)
  • 0.158 g/L/h (Escherichia coli ILE03 (pTacilvAIH, pBRthrABCygaZH) in Fed-Batch)
  Substrate yield
  • 0.14 g/g of sustrate (Escherichia coli ILE03 (pTacilvAIH, pBRthrABCygaZH) in Fed-Batch)
• acetate
  Titer
  • 12.61 g/L (Escherichia coli ILE03 (pTacilvAIH, pBRthrABCygaZH) in Fed-Batch)
  • 11.34 g/L (Escherichia coli ILE03 (pTacilvAIH, pBRthrABCygaZH) in Fed-Batch)