José Luis Báez‐Viveros, Joel Osuna, Georgina Hernández‐Chávez, Xavier Soberón, Francisco Bolívar & Guillermo Gosset (2004). Metabolic engineering and protein directed evolution increase the yield of L‐phenylalanine synthesized from glucose in Escherichia coli. Biotechnology & Bioengineering.

Strains

• Escherichia coli JM101
• Escherichia coli PB12
• Escherichia coli AB3248
• Escherichia coli JM101-fbr
• Escherichia coli JM101-ev1
• Escherichia coli JM101-ev2
• Escherichia coli PB12-fbr
• Escherichia coli PB12-ev1
• Escherichia coli PB12-ev2

Plasmids

• pRW300
• pCLtkt
• pJLBaroG^fbr
• pTrcpheA^fbr
• pTrcpheA^ev1
• PtrcpheA^ev2
• pACYC184
• pTrc99A

Metabolite production

• L-phenylalanine
  Substrate yield
  • 0.33 g/g of sustrate (Escherichia coli PB12-ev2 in Flask)
  • 0.05 g/g of sustrate (Escherichia coli JM101-fbr in Flask)
  • 0.14 g/g of sustrate (Escherichia coli PB12-fbr in Flask)
  • 0.21 g/g of sustrate (Escherichia coli JM101-ev1 in Flask)
  • 0.22 g/g of sustrate (Escherichia coli JM101-ev2 in Flask)
  • 0.33 g/g of sustrate (Escherichia coli PB12-ev1 in Flask)
  Titer
  • 0.16 g/L (Escherichia coli PB12-fbr in Flask)
  • 0.1 g/L (Escherichia coli JM101-fbr in Flask)
  Biomass-specific productivity
  • 38.0 mg/g DCW/h (Escherichia coli JM101-ev1 in Flask)
  • 36.0 mg/g DCW/h (Escherichia coli JM101-ev2 in Flask)
  • 38.0 mg/g DCW/h (Escherichia coli PB12-ev1 in Flask)
  • 40.6 mg/g DCW/h (Escherichia coli PB12-ev2 in Flask)
• 3-dehydroshikimate
  Titer
  • 0.054 g/L (Escherichia coli PB12-ev2 in Flask)
  • 0.15 g/L (Escherichia coli PB12-fbr in Flask)