Fuqiang Song, Zhijie Qin, Kun Qiu, Zhongshi Huang, Lian Wang, Heng Zhang, Xiaoyu Shan, Hao Meng, Xirong Liu & Jingwen Zhou (2024). Development of a vitamin B5 hyperproducer in Escherichia coli by multiple metabolic engineering. Metabolic Engineering.

Strains

• Escherichia coli JM109
• Escherichia coli PA100
• Escherichia coli PA101
• Escherichia coli PA102
• Escherichia coli PA104
• Escherichia coli PA105
• Escherichia coli PA106
• Escherichia coli PA107
• Escherichia coli PA108
• Escherichia coli PA109
• Escherichia coli PA110
• Escherichia coli PA111
• Escherichia coli PA111 pEc
• Escherichia coli PA111 pCg
• Escherichia coli PA111 pBs
• Escherichia coli PA112
• Escherichia coli PA113
• Escherichia coli PA114
• Escherichia coli PA115
• Escherichia coli PA116
• Escherichia coli PA117
• Escherichia coli PA118
• Escherichia coli PA119
• Escherichia coli PA120
• Escherichia coli PA121
• Escherichia coli PA122
• Escherichia coli PA123
• Escherichia coli PA124
• Escherichia coli PA125
• Escherichia coli PA126
• Escherichia coli PA127
• Escherichia coli PA128
• Escherichia coli PA129
• Escherichia coli PA130
• Escherichia coli PA131
• Escherichia coli PA107pA
• Escherichia coli PA107pC
• Escherichia coli PA107pF
• Escherichia coli PA107pR
• Escherichia coli PA132
• Escherichia coli PA103
• Escherichia coli MG1655

Plasmids

• pACYC-panBC_cgl
• pACYCDuet-panBC^fbr
• pACYC-panBC_bsu
• pACYC-panBC^fbr-ompA
• pACYC-panBC^fbr-ompC
• pACYC-panBC^fbr-ompF
• pACYC-panBC^fbr-tolR
• pACYCDuet-1

Metabolite production

• (R)-pantothenate
  Titer
  • 83.26 g/L (Escherichia coli PA132 in Fed-Batch)
  • 0.94 g/L (Escherichia coli PA109 in Flask)
  • 3.83 g/L (Escherichia coli PA113 in Flask)
  • 4.93 g/L (Escherichia coli PA125 in Flask)
  • 70.45 g/L (Escherichia coli PA132 in Fed-Batch)
  • 58.76 g/L (Escherichia coli PA117 in Fed-Batch)
  • 0.285 g/L (Escherichia coli PA101 in Flask)
  • 0.314 g/L (Escherichia coli PA102 in Flask)
  • 0.285 g/L (Escherichia coli PA103 in Flask)
  • 0.33 g/L (Escherichia coli PA104 in Flask)
  • 0.36 g/L (Escherichia coli PA105 in Flask)
  • 0.44 g/L (Escherichia coli PA107 in Flask)
  • 0.5 g/L (Escherichia coli PA107 in Flask)
  • 0.81 g/L (Escherichia coli PA107 in Flask)
  • 1.21 g/L (Escherichia coli PA111 in Flask)
  • 1.225 g/L (Escherichia coli PA111 pEc in Flask)
  • 3.71 g/L (Escherichia coli PA111 pEc in Flask)
  • 2.766 g/L (Escherichia coli PA111 pCg in Flask)
  • 0.828 g/L (Escherichia coli PA111 pCg in Flask)
  • 0.74 g/L (Escherichia coli PA111 pBs in Flask)
  • 2.722 g/L (Escherichia coli PA111 pBs in Flask)
  • 1.05 g/L (Escherichia coli PA111 in Flask)
  • 1.19 g/L (Escherichia coli PA111 in Flask)
  • 1.135 g/L (Escherichia coli PA111 in Flask)
  • 3.2 g/L (Escherichia coli PA111 pEc in Flask)
  • 3.672 g/L (Escherichia coli PA111 pEc in Flask)
  • 3.562 g/L (Escherichia coli PA111 pEc in Flask)
  • 1.466 g/L (Escherichia coli PA112 in Flask)
  • 2.238 g/L (Escherichia coli PA112 in Flask)
  • 3.727 g/L (Escherichia coli PA112 in Flask)
  • 3.81 g/L (Escherichia coli PA113 in Flask)
  • 3.727 g/L (Escherichia coli PA113 in Flask)
  • 2.762 g/L (Escherichia coli PA113 in Flask)
  • 3.87 g/L (Escherichia coli PA113 in Flask)
  • 3.75 g/L (Escherichia coli PA114 in Flask)
  • 3.53 g/L (Escherichia coli PA115 in Flask)
  • 3.89 g/L (Escherichia coli PA116 in Flask)
  • 4.27 g/L (Escherichia coli PA117 in Flask)
  • 3.845 g/L (Escherichia coli PA118 in Flask)
  • 4.108 g/L (Escherichia coli PA119 in Flask)
  • 3.379 g/L (Escherichia coli PA120 in Flask)
  • 4.02 g/L (Escherichia coli PA121 in Flask)
  • 3.99 g/L (Escherichia coli PA122 in Flask)
  • 4.21 g/L (Escherichia coli PA119 in Flask)
  • 4.33 g/L (Escherichia coli PA119 in Flask)
  • 4.82 g/L (Escherichia coli PA123 in Flask)
  • 4.443 g/L (Escherichia coli PA123 in Flask)
  • 3.569 g/L (Escherichia coli PA124 in Flask)
  • 3.74 g/L (Escherichia coli PA124 in Flask)
  • 4.85 g/L (Escherichia coli PA125 in Flask)
  • 0.035 g/L (Escherichia coli PA126 in Flask)
  • 0.461 g/L (Escherichia coli PA103 in Flask)
  • 0.564 g/L (Escherichia coli PA107pA in Flask)
  • 1.45 g/L (Escherichia coli PA107pC in Flask)
  • 0.978 g/L (Escherichia coli PA107pR in Flask)
  • 0.506 g/L (Escherichia coli PA127 in Flask)
  • 0.499 g/L (Escherichia coli PA128 in Flask)
  • 0.598 g/L (Escherichia coli PA129 in Flask)
  • 1.09 g/L (Escherichia coli PA130 in Flask)
  • 0.89 g/L (Escherichia coli PA107pF in Flask)
  • 4.627 g/L (Escherichia coli PA125 in Flask)
  • 4.566 g/L (Escherichia coli PA131 in Flask)
  • 5.04 g/L (Escherichia coli PA132 in Flask)
  • 0.26 g/L (Escherichia coli PA106 in Flask)
  Volumetric productivity
  • 0.05 g/L/h (Escherichia coli PA112 in Flask)
  • 1.74 g/L/h (Escherichia coli PA132 in Fed-Batch)
  • 0.08 g/L/h (Escherichia coli PA111 pEc in Flask)
  Substrate yield
  • 0.39 g/g of sustrate (Escherichia coli PA132 in Fed-Batch)
  Biomass-specific productivity
  • 0.39 g/L/OD₆₀₀ (Escherichia coli PA107pA in Flask)
  • 0.116 g/L/OD₆₀₀ (Escherichia coli PA130 in Flask)
  • 0.036 g/L/OD₆₀₀ (Escherichia coli PA129 in Flask)
  • 0.031 g/L/OD₆₀₀ (Escherichia coli PA128 in Flask)
  • 0.035 g/L/OD₆₀₀ (Escherichia coli PA127 in Flask)
  • 0.07 g/L/OD₆₀₀ (Escherichia coli PA107pR in Flask)
  • 0.062 g/L/OD₆₀₀ (Escherichia coli PA107pF in Flask)
  • 0.104 g/L/OD₆₀₀ (Escherichia coli PA107pC in Flask)
  • 0.203 g/L/OD₆₀₀ (Escherichia coli PA122 in Flask)
  • 0.199 g/L/OD₆₀₀ (Escherichia coli PA121 in Flask)
  • 0.189 g/L/OD₆₀₀ (Escherichia coli PA120 in Flask)
  • 0.019 g/L/OD₆₀₀ (Escherichia coli PA101 in Flask)
  • 0.241 g/L/OD₆₀₀ (Escherichia coli PA119 in Flask)
  • 0.024 g/L/OD₆₀₀ (Escherichia coli PA102 in Flask)
  • 0.204 g/L/OD₆₀₀ (Escherichia coli PA118 in Flask)
  • 0.179 g/L/OD₆₀₀ (Escherichia coli PA113 in Flask)
  • 0.179 g/L/OD₆₀₀ (Escherichia coli PA114 in Flask)
  • 0.161 g/L/OD₆₀₀ (Escherichia coli PA115 in Flask)
  • 0.188 g/L/OD₆₀₀ (Escherichia coli PA116 in Flask)
  • 0.211 g/L/OD₆₀₀ (Escherichia coli PA117 in Flask)
  • 0.038 g/L/OD₆₀₀ (Escherichia coli PA111 pBs in Flask)
  • 0.172 g/L/OD₆₀₀ (Escherichia coli PA111 pBs in Flask)
  • 0.048 g/L/OD₆₀₀ (Escherichia coli PA111 pCg in Flask)
  • 0.169 g/L/OD₆₀₀ (Escherichia coli PA111 pCg in Flask)
  • 0.058 g/L/OD₆₀₀ (Escherichia coli PA111 pEc in Flask)
  • 0.211 g/L/OD₆₀₀ (Escherichia coli PA111 pEc in Flask)
  • 0.054 g/L/OD₆₀₀ (Escherichia coli PA110 in Flask)
  • 0.025 g/L/OD₆₀₀ (Escherichia coli PA110 in Flask)
  • 0.639 g/L/OD₆₀₀ (Escherichia coli PA109 in Flask)
  • 0.033 g/L/OD₆₀₀ (Escherichia coli PA109 in Flask)
  • 0.051 g/L/OD₆₀₀ (Escherichia coli PA108 in Flask)
  • 0.027 g/L/OD₆₀₀ (Escherichia coli PA108 in Flask)
  • 0.035 g/L/OD₆₀₀ (Escherichia coli PA107 in Flask)
  • 0.026 g/L/OD₆₀₀ (Escherichia coli PA107 in Flask)
  • 0.049 g/L/OD₆₀₀ (Escherichia coli PA107 in Flask)
  • 0.033 g/L/OD₆₀₀ (Escherichia coli PA106 in Flask)
  • 0.028 g/L/OD₆₀₀ (Escherichia coli PA105 in Flask)
  • 0.026 g/L/OD₆₀₀ (Escherichia coli PA104 in Flask)
  • 0.025 g/L/OD₆₀₀ (Escherichia coli PA103 in Flask)
• acetate
  Titer
  • 4.5 g/L (Escherichia coli PA105 in Flask)
  • 1.52 g/L (Escherichia coli PA106 in Flask)
  • 2.05 g/L (Escherichia coli PA107 in Flask)