Björn-Johannes Harder, Katja Bettenbrock & Steffen Klamt (2016). Model-based metabolic engineering enables high yield itaconic acid production by Escherichia coli. Metabolic Engineering.

Strains

• Escherichia coli ita1
• Escherichia coli ita4
• Escherichia coli ita6
• Escherichia coli ita12
• Escherichia coli ita19
• Escherichia coli ita23
• Escherichia coli MG1655

Plasmids

• pCadCs

Metabolite production

• itaconate
  Titer
  • 32.0 g/L (Escherichia coli ita23 in Fed-Batch)
  • 0.11 g/L (Escherichia coli ita1 in Flask)
  • 0.11 g/L (Escherichia coli ita4 in Flask)
  • 0.02 g/L (Escherichia coli ita6 in Flask)
  • 0.02 g/L (Escherichia coli ita12 in Flask)
  • 0.06 g/L (Escherichia coli ita19 in Flask)
  • 2.27 g/L (Escherichia coli ita23 in Flask)
  Substrate yield
  • 0.68 mol/mol of substrate (Escherichia coli ita23 in Fed-Batch)
  • 0.039 mol/mol of substrate (Escherichia coli ita1 in Flask)
  • 0.036 mol/mol of substrate (Escherichia coli ita4 in Flask)
  • 0.007 mol/mol of substrate (Escherichia coli ita6 in Flask)
  • 0.006 mol/mol of substrate (Escherichia coli ita12 in Flask)
  • 0.02 mol/mol of substrate (Escherichia coli ita19 in Flask)
  • 0.767 mol/mol of substrate (Escherichia coli ita23 in Flask)
  Volumetric productivity
  • 0.45 g/L/h (Escherichia coli ita23 in Fed-Batch)
• acetate
  Substrate yield
  • 0.03 mol/mol of substrate (Escherichia coli ita19 in Flask)