Xianghao Wu, D. Brisbane Tovilla‐Coutiño & Mark A. Eiteman (2020). Engineered citrate synthase improves citramalic acid generation in Escherichia coli. Biotechnology & Bioengineering.

Strains

• Escherichia coli MEC568
• Escherichia coli MEC569
• Escherichia coli MEC613
• Escherichia coli MEC624
• Escherichia coli MEC626
• Escherichia coli MEC648
• Escherichia coli MEC649
• Escherichia coli MEC654
• Escherichia coli MEC626/pZE12-cimA
• Escherichia coli MEC613/pZE12-cimA
• Escherichia coli MEC649/pZE12-cimA
• Escherichia coli MEC624/pZE12-cimA
• Escherichia coli MEC654/pZE12-cimA
• Escherichia coli MEC648/pZE12-cimA

Plasmids

• pZE12-cimA

Metabolite production

• (R)-citramalate
  Titer
  • 60.0 g/L (Escherichia coli MEC626/pZE12-cimA in Fed-Batch)
  • 1.72 g/L (Escherichia coli MEC613/pZE12-cimA in Flask)
  • 1.81 g/L (Escherichia coli MEC626/pZE12-cimA in Flask)
  • 0.84 g/L (Escherichia coli MEC649/pZE12-cimA in Flask)
  • 0.46 g/L (Escherichia coli MEC624/pZE12-cimA in Flask)
  • 1.23 g/L (Escherichia coli MEC654/pZE12-cimA in Flask)
  • 0.98 g/L (Escherichia coli MEC648/pZE12-cimA in Flask)
  • 4.7 g/L (Escherichia coli MEC613/pZE12-cimA in Batch)
  • 11.0 g/L (Escherichia coli MEC626/pZE12-cimA in Batch)
  Substrate yield
  • 0.53 g/g of sustrate (Escherichia coli MEC626/pZE12-cimA in Fed-Batch)
  • 0.47 g/g of sustrate (Escherichia coli MEC613/pZE12-cimA in Flask)
  • 0.57 g/g of sustrate (Escherichia coli MEC626/pZE12-cimA in Flask)
  • 0.49 g/g of sustrate (Escherichia coli MEC648/pZE12-cimA in Flask)
  • 0.37 g/g of sustrate (Escherichia coli MEC626/pZE12-cimA in Batch)
  • 0.16 g/g of sustrate (Escherichia coli MEC613/pZE12-cimA in Flask)
  • 0.4 g/g of sustrate (Escherichia coli MEC649/pZE12-cimA in Flask)
  • 0.432 g/g of sustrate (Escherichia coli MEC624/pZE12-cimA in Flask)
  • 0.5 g/g of sustrate (Escherichia coli MEC654/pZE12-cimA in Flask)
• acetate
  Titer
  • 0.75 g/L (Escherichia coli MEC626/pZE12-cimA in Batch)
  • 0.0 g/L (Escherichia coli MEC613/pZE12-cimA in Batch)
  • 3.1 g/L (Escherichia coli MEC626/pZE12-cimA in Fed-Batch)
• (R)-lactate
  Titer
  • 1.3 g/L (Escherichia coli MEC626/pZE12-cimA in Fed-Batch)