Wang, HD., Xu, JZ. & Zhang, WG. Metabolic engineering of Escherichia coli for efficient production of l-arginine. Appl Microbiol Biotechnol 106, 5603–5613 (2022).

Strains

• Escherichia coli DH5α
• Escherichia coli A1
• Escherichia coli A2
• Escherichia coli A3
• Escherichia coli A4
• Escherichia coli A5
• Escherichia coli A6
• Escherichia coli A7
• Escherichia coli MG1655

Metabolite production

• L-arginine
  Titer
  • 2.9 g/L (Escherichia coli A1 in Flask)
  • 0.0 g/L (Escherichia coli MG1655 in Flask)
  • 2.82 g/L (Escherichia coli A2 in Flask)
  • 3.03 g/L (Escherichia coli A3 in Flask)
  • 4.2 g/L (Escherichia coli A4 in Flask)
  • 3.3 g/L (Escherichia coli A5 in Flask)
  • 12.8 g/L (Escherichia coli A6 in Flask)
  • 16.9 g/L (Escherichia coli A7 in Flask)
  • 61.2 g/L (Escherichia coli A6 in Fed-Batch)
  • 70.1 g/L (Escherichia coli A7 in Fed-Batch)
  Substrate yield
  • 0.0 g/g of sustrate (Escherichia coli MG1655 in Flask)
  • 0.209 g/g of sustrate (Escherichia coli A7 in Flask)
  • 0.326 g/g of sustrate (Escherichia coli A7 in Fed-Batch)
  • 0.289 g/g of sustrate (Escherichia coli A6 in Fed-Batch)
  • 0.048 g/g of sustrate (Escherichia coli A1 in Flask)
  • 0.055 g/g of sustrate (Escherichia coli A3 in Flask)
  • 0.05 g/g of sustrate (Escherichia coli A2 in Flask)
  • 0.07 g/g of sustrate (Escherichia coli A4 in Flask)
  • 0.055 g/g of sustrate (Escherichia coli A5 in Flask)
  • 0.16 g/g of sustrate (Escherichia coli A6 in Flask)
  Volumetric productivity
  • 1.17 g/L/h (Escherichia coli A7 in Fed-Batch)
• acetate
  Titer
  • 12.4 g/L (Escherichia coli A6 in Flask)
  • 1.8 g/L (Escherichia coli A7 in Flask)
  • 15.7 g/L (Escherichia coli A7 in Fed-Batch)
  • 12.43 g/L (Escherichia coli A6 in Fed-Batch)