Vo, Toan Minh & Park, Sunghoon. Metabolic engineering of Escherichia coli W3110 for efficient production of homoserine from glucose. Metabolic Engineering. 2022, 73, 104-113.

Strains

Plasmids

Metabolite production

L-homoserine
- 0.0 mM (Escherichia coli W3110 in Flask)
- 0.0 mM (Escherichia coli W-H4 in Flask)
- 0.52 mM (Escherichia coli W-H7 in Flask)
- 8.06 mM (Escherichia coli W-H7/pM1/pR1 in Flask)
- 36.0 mM (Escherichia coli W-9/pM1/pR1 in Flask)
- 2.17 mM (Escherichia coli W-8/pM1/pR1 in Flask)
- 43.0 mM (Escherichia coli W-10/pM1/pR1 in Flask)
- 63.7 mM (Escherichia coli W-14/pM1/pR1 in Flask)
- 61.1 mM (Escherichia coli W-11/pM1/pR1 in Flask)
- 48.64 mM (Escherichia coli W-13/pM1/pR1 in Flask)
- 53.37 mM (Escherichia coli W-12/pM1/pR1 in Flask)
- 77.3 mM (Escherichia coli W-H15/pM1/pR1 in Flask)
- 93.4 mM (Escherichia coli W-H17/pM2/pR2 in Flask)
- 89.1 mM (Escherichia coli W-H17/pM2/pR1 in Flask)
- 53.7 mM (Escherichia coli W-H17/pM1/pR1 in Flask)
- 46.0 mM (Escherichia coli W-H15/pM2/pR2 in Flask)
- 52.0 mM (Escherichia coli W-H16/pM2/pR2 in Flask)
- 12.3 g/L (Escherichia coli W-H18/pM2/pR1 in Flask)
- 12.5 g/L (Escherichia coli W-H18/pM2/pR1 in Flask)
- 12.4 g/L (Escherichia coli W-H19/pM2/pR1 in Flask)
- 10.5 g/L (Escherichia coli W-H19/pM2/pR1 in Flask)
- 12.7 g/L (Escherichia coli W-H20/pM2/pR1 in Flask)
- 10.8 g/L (Escherichia coli W-H20/pM2/pR1 in Flask)
- 110.8 g/L (Escherichia coli W-H18/pM2/pR1 in Fed-Batch)
- 0.005 mol/mol of substrate (Escherichia coli W-H7 in Flask)
- 0.0778 mol/mol of substrate (Escherichia coli W-H7/pM1/pR1 in Flask)
- 0.32 mol/mol of substrate (Escherichia coli W-9/pM1/pR1 in Flask)
- 0.37 mol/mol of substrate (Escherichia coli W-10/pM1/pR1 in Flask)
- 0.59 mol/mol of substrate (Escherichia coli W-H17/pM2/pR2 in Flask)
- 0.59 mol/mol of substrate (Escherichia coli W-H17/pM2/pR1 in Flask)
- 0.35 mol/mol of substrate (Escherichia coli W-H17/pM1/pR1 in Flask)
- 0.68 mol/mol of substrate (Escherichia coli W-H18/pM2/pR1 in Flask)
- 0.68 mol/mol of substrate (Escherichia coli W-H18/pM2/pR1 in Flask)
- 0.69 mol/mol of substrate (Escherichia coli W-H19/pM2/pR1 in Flask)
- 0.57 mol/mol of substrate (Escherichia coli W-H19/pM2/pR1 in Flask)
- 0.7 mol/mol of substrate (Escherichia coli W-H20/pM2/pR1 in Flask)
- 0.58 mol/mol of substrate (Escherichia coli W-H20/pM2/pR1 in Flask)
- 0.96 mol/mol of substrate (Escherichia coli W-H18/pM2/pR1 in Fed-Batch)
- 0.59 g/L/h (Escherichia coli W-H18/pM2/pR1 in Flask)
- 0.73 g/L/h (Escherichia coli W-H18/pM2/pR1 in Flask)
- 0.59 g/L/h (Escherichia coli W-H19/pM2/pR1 in Flask)
- 0.55 g/L/h (Escherichia coli W-H19/pM2/pR1 in Flask)
- 0.6 g/L/h (Escherichia coli W-H20/pM2/pR1 in Flask)
- 0.63 g/L/h (Escherichia coli W-H20/pM2/pR1 in Flask)
- 1.82 g/L/h (Escherichia coli W-H18/pM2/pR1 in Fed-Batch)
acetate
- 6.2 mM (Escherichia coli W-H7/pM1/pR1 in Flask)
- 1.25 mM (Escherichia coli W-8/pM1/pR1 in Flask)
- 5.64 mM (Escherichia coli W-9/pM1/pR1 in Flask)
- 4.28 mM (Escherichia coli W-10/pM1/pR1 in Flask)