Arense, Paula; Bernal, Vicente; Charlier, Daniël; Iborra, José Luis; Foulquié-Moreno, Maria Remedios & Cánovas, Manuel. Metabolic engineering for high yielding L(-)-carnitine production in Escherichia coli. Microbial Cell Factories. 2013, 12(1).

Strains

• Escherichia coli BW25113
• Escherichia coli BW25113 ∆aceK
• Escherichia coli BW25113 ∆aceA
• Escherichia coli BW25113 ∆aceAK
• Escherichia coli BW25113 ∆caiA
• Escherichia coli BW25113 ∆pcaiF caiF-p8
• Escherichia coli BW25113 ∆pcai cai-p37
• Escherichia coli BW25113 ∆aceK ∆pcai cai-p37
• Escherichia coli BW25113 ∆aceK ∆caiA ∆pcai cai-p37
• Escherichia coli BL21(DE3)

Metabolite production

• L-carnitine
  Titer
  • 14.23 mM (Escherichia coli BW25113 in Flask)
  • 16.83 mM (Escherichia coli BW25113 ∆aceK in Flask)
  • 19.214 mM (Escherichia coli BW25113 ∆pcaiF caiF-p8 in Flask)
  • 22.24 mM (Escherichia coli BW25113 ∆aceK ∆pcai cai-p37 in Flask)
  • 28.3 mM (Escherichia coli BW25113 ∆aceK ∆caiA ∆pcai cai-p37 in Flask)
  • 16.62 mM (Escherichia coli BW25113 in Flask)
  • 21.09 mM (Escherichia coli BW25113 ∆aceK in Flask)
  • 21.53 mM (Escherichia coli BW25113 ∆pcaiF caiF-p8 in Flask)
  • 24.21 mM (Escherichia coli BW25113 ∆pcai cai-p37 in Flask)
  • 24.66 mM (Escherichia coli BW25113 ∆aceK ∆pcai cai-p37 in Flask)
  • 0.0 mM (Escherichia coli BW25113 in Flask)
  • 0.0 mM (Escherichia coli BW25113 in Flask)
  • 0.0 mM (Escherichia coli BW25113 ∆aceK in Flask)
  • 0.0 mM (Escherichia coli BW25113 ∆aceK in Flask)
  • 0.0 mM (Escherichia coli BW25113 ∆caiA in Flask)
  • 0.0 mM (Escherichia coli BW25113 ∆caiA in Flask)
  • 4.55 mM (Escherichia coli BW25113 ∆pcaiF caiF-p8 in Flask)
  • 7.27 mM (Escherichia coli BW25113 ∆pcaiF caiF-p8 in Flask)
  • 19.54 mM (Escherichia coli BW25113 ∆pcai cai-p37 in Flask)
  • 20.45 mM (Escherichia coli BW25113 ∆pcai cai-p37 in Flask)
  • 25.0 mM (Escherichia coli BW25113 ∆aceK ∆pcai cai-p37 in Flask)
  • 25.9 mM (Escherichia coli BW25113 ∆aceK ∆pcai cai-p37 in Flask)
  • 24.09 mM (Escherichia coli BW25113 ∆aceK ∆caiA ∆pcai cai-p37 in Flask)
  • 20.7 mM (Escherichia coli BW25113 ∆caiA in Flask)
  • 21.7 mM (Escherichia coli BW25113 ∆pcai cai-p37 in Flask)
  • 28.7 mM (Escherichia coli BW25113 ∆caiA in Flask)
  • 46.0 mM (Escherichia coli BW25113 ∆aceK ∆caiA ∆pcai cai-p37 in Flask)
  • 31.2 mM (Escherichia coli BW25113 ∆aceK ∆caiA ∆pcai cai-p37 in Flask)