Kazuhiko Tabata & Shin-Ichi Hashimoto (2004). Production of mevalonate by a metabolically-engineered Escherichia coli. Biotechnology Letters.

Strains

• Escherichia coli NM522
• Escherichia coli XL1-Blue/pMVA2
• Escherichia coli NM522/pMVA2

Plasmids

• pTrS30
• pMVA1
• pMVA2

Metabolite production

• (R)-mevalonate
  Titer
  • 47.0 g/L (Escherichia coli XL1-Blue/pMVA2 in Fed-Batch)
  • 25.0 g/L (Escherichia coli NM522/pMVA2 in Fed-Batch)
• acetate
  Titer
  • 0.0 g/L (Escherichia coli XL1-Blue/pMVA2 in Fed-Batch)
  • 13.0 g/L (Escherichia coli NM522/pMVA2 in Fed-Batch)