Darmawi Juminaga, Edward E. K. Baidoo, Alyssa M. Redding-Johanson, Tanveer S. Batth, Helcio Burd, Aindrila Mukhopadhyay, Christopher J. Petzold & Jay D. Keasling (2011). Modular Engineering of l-Tyrosine Production in Escherichia coli. Applied and Environmental Microbiology.
Strains
- Escherichia coli DPD4193
- Escherichia coli MG1655
- Escherichia coli B (pS0, pY1)
- Escherichia coli A (pS0, pY0)
- Escherichia coli C (pS4, pY1)
- Escherichia coli D (pS5, pY1)
- Escherichia coli E (pS4, pY2)
- Escherichia coli F (pS4, pY3)
- Escherichia coli G (pS5, pY3)
Plasmids
Metabolite production
-
L-tyrosine
-
Titer
- 0.18 g/L (Escherichia coli DPD4193 in Flask)
- 55.0 g/L (Escherichia coli DPD4193 in Fed-Batch)
- 1310.0 mg/L (Escherichia coli G (pS5, pY3) in Flask)
- 2169.0 mg/L (Escherichia coli F (pS4, pY3) in Flask)
- 746.0 mg/L (Escherichia coli A (pS0, pY0) in Flask)
- 897.0 mg/L (Escherichia coli B (pS0, pY1) in Flask)
- 1150.0 mg/L (Escherichia coli C (pS4, pY1) in Flask)
- 1219.0 mg/L (Escherichia coli D (pS5, pY1) in Flask)
- 908.0 mg/L (Escherichia coli E (pS4, pY2) in Flask) Substrate yield
- 0.09 g/g of sustrate (Escherichia coli DPD4193 in Flask) Biomass-specific yield
- 1617.0 mg/g DCW (Escherichia coli G (pS5, pY3) in Flask)
- 2645.0 mg/g DCW (Escherichia coli F (pS4, pY3) in Flask)
- 921.0 mg/g DCW (Escherichia coli A (pS0, pY0) in Flask)
- 944.0 mg/g DCW (Escherichia coli B (pS0, pY1) in Flask)
- 1307.0 mg/g DCW (Escherichia coli C (pS4, pY1) in Flask)
- 1451.0 mg/g DCW (Escherichia coli D (pS5, pY1) in Flask)
- 987.0 mg/g DCW (Escherichia coli E (pS4, pY2) in Flask)