Wang, Hai-De; Xu, Jian-Zhong & Zhang, Wei-Guo. Reduction of acetate synthesis, enhanced arginine export, and supply of precursors, cofactors, and energy for improved synthesis of L-arginine by Escherichia coli. Applied Microbiology and Biotechnology. 2023, 107(11), 3593-3603.
Strains
- Escherichia coli A7
- Escherichia coli A1
- Escherichia coli A8
- Escherichia coli A10
- Escherichia coli A11
- Escherichia coli A12
- Escherichia coli A13
- Escherichia coli A14
- Escherichia coli A15
- Escherichia coli A16
- Escherichia coli A17
- Escherichia coli A18
- Escherichia coli A19
- Escherichia coli A20
- Escherichia coli A21
- Escherichia coli A9
- Escherichia coli MG1655
Metabolite production
-
L-arginine
-
Titer
- 89.7 g/L (Escherichia coli A21 in Fed-Batch)
- 26.7 g/L (Escherichia coli A20 in Flask)
- 27.3 g/L (Escherichia coli A21 in Flask)
- 17.6 g/L (Escherichia coli A10 in Flask)
- 22.4 g/L (Escherichia coli A14 in Flask)
- 18.7 g/L (Escherichia coli A12 in Flask)
- 19.6 g/L (Escherichia coli A13 in Flask)
- 25.9 g/L (Escherichia coli A19 in Flask) Volumetric productivity
- 1.495 g/L/h (Escherichia coli A21 in Fed-Batch) Substrate yield
- 0.377 g/g of sustrate (Escherichia coli A21 in Fed-Batch)
-
acetate
-
Titer
- 3.2 g/L (Escherichia coli A21 in Fed-Batch)
- 0.25 g/L (Escherichia coli A10 in Flask)