Koirala, Niranjan; Pandey, Ramesh Prasad; Thuan, Nguyen Huy; Ghimire, Gopal Prasad; Jung, Hye Jin; Oh, Tae‐Jin & Sohng, Jae Kyung (2019). Metabolic engineering of Escherichia coli for the production of isoflavonoid‐4′‐O‐methoxides and their biological activities. Biotechnology and Applied Biochemistry, 66(4), 484-493.

Strains

• Escherichia coli BL21(DE3)
• Escherichia coli XL1-Blue
• Escherichia coli M
• Escherichia coli MS

Plasmids

• pRSF-Duet-SaOMT2
• pRSF-Duet-SaOMT2-metK

Metabolite production

• prunetin
  Titer
  • 90.5 µM (Escherichia coli MS in Flask)
  • 112.5 µM (Escherichia coli MS in Flask)
  • 164.25 µM (Escherichia coli MS in Fed-Batch)
• isoformononetin
  Titer
  • 184.45 µM (Escherichia coli MS in Flask)
  • 188.0 µM (Escherichia coli MS in Flask)
  • 382.5 µM (Escherichia coli MS in Fed-Batch)