Escherichia coli MS

Synonyms

Escherichia coli BL21(DE3)/pRSF-Duet-SaOMT2-metK

Ancestors

Genotype with respect to wild type
F− dcm gal ompT hsdS(rB- mB-) | F− ompT hsdSB (rB−mB−) gal dcm λ(DE3) | Plasmid pRSF-Duet-SaOMT2-metK added

Bars (|) indicate differences between strains.


Plasmids

Modifications
Origin Gene Organism Modification
Overexpression
Overexpression

Production
Metabolites Production type Production Biomass Carbon source Time Scale Ref.
prunetin Titer 90.5 µM Flask [82]
isoformononetin Titer 184.45 µM Flask [82]
prunetin Titer 112.5 µM Flask [82]
isoformononetin Titer 188.0 µM Flask [82]
isoformononetin Titer 382.5 µM 48 h Fed-Batch [82]
prunetin Titer 164.25 µM 48 h Fed-Batch [82]

* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References