Dekishima, Yasumasa; Lan, Ethan I.; Shen, Claire R.; Cho, Kwang Myung & Liao, James C. (2011). Extending Carbon Chain Length of 1-Butanol Pathway for 1-Hexanol Synthesis from Glucose by Engineered Escherichia coli. Journal of the American Chemical Society, 133(30), 11399-11401.

Strains

• Escherichia coli BL21(DE3)
• Escherichia coli XL1-Blue
• Escherichia coli JCL166
• Escherichia coli JCL299
• Escherichia coli JCL166/pEL11/pEL102
• Escherichia coli JCL299/pEL11/pEL102
• Escherichia coli JCL299/pEL11/pEL102/pCS138
• Escherichia coli JCL167

Plasmids

• pCDFDuet-1
• pCDFDuet-1_bktB
• pCDFDuet-1_hbd
• pCDFDuet-1_crt
• pCDFDuet-1_TdTer
• pCDFDuet-1_EgTer
• pIM8
• pEL11
• pEL100
• pEL102
• pCS138
• pCS38_adhE2

Metabolite production

• 1-hexanol
  Titer
  • 23.0 mg/L (Escherichia coli JCL166/pEL11/pEL102 in Flask)
  • 27.0 mg/L (Escherichia coli JCL299/pEL11/pEL102 in Flask)
  • 47.0 mg/L (Escherichia coli JCL299/pEL11/pEL102/pCS138 in Fed-Batch)
• butan-1-ol
  Titer
  • 6.5 g/L (Escherichia coli JCL299/pEL11/pEL102/pCS138 in Fed-Batch)
  • 5.1 g/L (Escherichia coli JCL299/pEL11/pEL102/pCS138 in Fed-Batch)
• ethanol
  Titer
  • 0.2 g/L (Escherichia coli JCL299/pEL11/pEL102/pCS138 in Fed-Batch)