Escherichia coli JCL299/pEL11/pEL102/pCS138
Ancestors
Genotype with respect to wild type
F+ (λ) | IacIq rrnBT14 ∆lacZWJ16 hsdR514 ∆araBADAH33 ∆rhaBADLD78 | F' [traD36, proAB+, lacIq ZDM15] | ΔldhA, ΔadhE, ΔfrdBC | Δpta | Plasmid pEL11 added | Plasmid pCS138 added | Plasmid pEL102 addedBars (|) indicate differences between strains.
Plasmids
-
pEL11 (PLlacO1::atoBEc-adhE2Ca-crtCa-hbdCa, ColE1 ori)
pEL102 (PLlacO1-bktB-terTd)
pCS138 (PLlacO1:: fdhCb pSC101 ori)
Modifications
| Origin | Gene | Organism | Modification |
|---|---|---|---|
|
Silencing
|
|||
|
Silencing
|
|||
|
Silencing
|
|||
|
Silencing
|
|||
|
Silencing
|
|||
|
Overexpression
|
|||
|
Overexpression
|
|||
|
Overexpression
|
|||
|
Overexpression
|
|||
|
Overexpression
|
|||
|
Overexpression
|
|||
|
Overexpression
|
Production
| Metabolites | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
|---|---|---|---|---|---|---|---|
| 1-hexanol | Titer | 47.0 mg/L | 48 h | Fed-Batch | [89] | ||
| butan-1-ol | Titer | 6.5 g/L | 68 h | Fed-Batch | [89] | ||
| butan-1-ol | Titer | 5.1 g/L | 48 h | Fed-Batch | [89] | ||
| ethanol | Titer | 0.2 g/L | Fed-Batch | [89] |
* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.