pgl

Escherichia coli

Sequence

                ATGAAGCAAACAGTTTATATCGCCAGCCCTGAGAGCCAGCAAATTCACGTCTGGAATCTGAATCATGAAGGCGCACTGACGCTGACACAGGTTGTCGATGTGCCGGGGCAGGTGCAGCCGATGGTGGTCAGCCCGGACAAACGTTATCTCTATGTTGGTGTTCGCCCTGAGTTTCGCGTCCTGGCGTATCGTATCGCCCCGGACGATGGCGCACTGACCTTTGCCGCAGAGTCTGCGCTGCCGGGTAGTCCGACGCATATTTCCACCGATCACCAGGGGCAGTTTGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGATCGCATTTGCCTGTTTACGGTCAGCGATGATGGTCATCTCGTGGCGCAGGACCCTGCGGAAGTGACCACCGTTGAAGGGGCCGGCCCGCGTCATATGGTATTCCATCCAAACGAACAATATGCGTATTGCGTCAATGAGTTAAACAGCTCAGTGGATGTCTGGGAACTGAAAGATCCGCACGGTAATATCGAATGTGTCCAGACGCTGGATATGATGCCGGAAAACTTCTCCGACACCCGTTGGGCGGCTGATATTCATATCACCCCGGATGGTCGCCATTTATACGCCTGCGACCGTACCGCCAGCCTGATTACCGTTTTCAGCGTTTCGGAAGATGGCAGCGTGTTGAGTAAAGAAGGCTTCCAGCCAACGGAAACCCAGCCGCGCGGCTTCAATGTTGATCACAGCGGCAAGTATCTGATTGCCGCCGGGCAAAAATCTCACCACATCTCGGTATACGAAATTGTTGGCGAGCAGGGGCTACTGCATGAAAAAGGCCGCTATGCGGTCGGGCAGGGACCAATGTGGGTGGTGGTTAACGCACACTAA

pgl encodes 6-phosphogluconolactonase (PGL), which catalyzes the conversion of 6-phosphogluconolactone to 6-phosphogluconate in the oxidative branch of the pentose phosphate pathway [341, 345]. Overexpression of pgl increases flux through the pentose phosphate pathway and NADPH availability, thereby enhancing biomass accumulation and riboflavin production [345]. By contrast, pgl is absent in Escherichia coli BL21 (DE3), which restricts oxidative pentose phosphate pathway flux and shifts carbon flow toward the non-oxidative branch, increasing phosphoribosyl pyrophosphate (PRPP) availability and improving inosine monophosphate (IMP) biosynthesis [341].

`Gene size:`
`Protein size:`
`Reactions`	R464
`Compounds affected`	riboflavin

Databases

`EraGene:`	2112061
`UniProt:`	P52697
`KEGG:`	eco:b0767