pTrc-BF1

Ancestors

All plasmids

pTrc99A

pTrc-BF1

Main features

P_trc-trpB^C1137G, P_tac-fmo_Ma

Selection marker:

Ampicillin

Modifications

Gene	Organism	Modification
*trpB*	Escherichia coli	Feedback insensitive
*fmo*	Methylophaga aminisulfidivorans	Overexpression

References

Jikun Du, Gongsoo Yang, Zi Wei Luo, Sang Yup Lee (2018). Metabolic engineering of Escherichia coli for the production of indirubin from glucose. Journal of Biotechnology.