Jikun Du, Gongsoo Yang, Zi Wei Luo, Sang Yup Lee (2018). Metabolic engineering of Escherichia coli for the production of indirubin from glucose. Journal of Biotechnology.
Strains
- Escherichia coli DH5α
- Escherichia coli W3110
- Escherichia coli IND2
- Escherichia coli IND4
- Escherichia coli IND5
- Escherichia coli W3110/pTrc-fmo
- Escherichia coli W3110/pTrc-cfmo
- Escherichia coli W3110/pTrcTF
- Escherichia coli W3110/pTrcTF1
- Escherichia coli IND2/pTrcTF1
- Escherichia coli IND2/pTrcTF1/pTac-G
- Escherichia coli IND2/pTrcTF1/pTac-GE1
- Escherichia coli IND2/pTrcTF1/pTac-GE2
- Escherichia coli IND2/pTrcTF1/pTac-GEL
- Escherichia coli IND4/pTrcTF1/pTac-GEL
- Escherichia coli IND5/pTrcTF1/pTac-GEL
Plasmids
- pEcmuloxC
- pJW168
- pKD46
- pMtrc9
- pTyr-a
- pTac15K
- pTrc99A
- pTrc-fmo
- pTrc-cfmo
- pTac-fmo
- pTrc-tnaA
- pTrc-trpBC1137G
- pTrcTF
- pTrcTF1
- pTrcTF2
- pTrc-BF
- pTrc-BF1
- pTrc-BF2
- pTac-G
- pTac-trpE
- pTac-E
- pTac-GE1
- pTac-GE2
- pTac-aroA
- pTac-aroB
- pTac-aroC
- pTac-aroD
- pTac-aroE
- pTac-aroK
- pTac-aroL
- pTac-ydiB
- pTacGEA
- pTacGEB
- pTacGEC
- pTacGED
- pTacGEE
- pTacGEK
- pTacGEL
- pTacGEY
- pTac-T
- pTac-GETD
- pTac-GETL
- pTac-GETY
Metabolite production
-
indigo
-
Titer
- 19.0 mg/L (Escherichia coli W3110/pTrc-fmo in Flask)
- 10.1 mg/L (Escherichia coli W3110/pTrc-cfmo in Flask)
- 0.0 mg/L (Escherichia coli W3110 in Flask)
- 35.5 mg/L (Escherichia coli W3110/pTrcTF in Flask)
- 39.3 mg/L (Escherichia coli W3110/pTrcTF1 in Flask)
- 44.2 mg/L (Escherichia coli IND2/pTrcTF1 in Flask)
- 60.8 mg/L (Escherichia coli IND2/pTrcTF1/pTac-G in Flask)
- 64.9 mg/L (Escherichia coli IND2/pTrcTF1/pTac-GE1 in Flask)
- 60.4 mg/L (Escherichia coli IND2/pTrcTF1/pTac-GE2 in Flask)
- 90.8 mg/L (Escherichia coli IND2/pTrcTF1/pTac-GEL in Flask)
- 104.0 mg/L (Escherichia coli IND4/pTrcTF1/pTac-GEL in Flask)
- 108.0 mg/L (Escherichia coli IND5/pTrcTF1/pTac-GEL in Flask)
- 0.629 g/L (Escherichia coli IND4/pTrcTF1/pTac-GEL in Fed-Batch)
- 0.64 g/L (Escherichia coli IND5/pTrcTF1/pTac-GEL in Fed-Batch)
-
Indirubin
-
Titer
- 0.033 g/L (Escherichia coli IND4/pTrcTF1/pTac-GEL in Fed-Batch)
- 0.056 g/L (Escherichia coli IND5/pTrcTF1/pTac-GEL in Fed-Batch)