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pTrcTF1

Ancestors
  • All plasmids
    • pTrc99A
      • pTrcTF1



Main features
Ptrc-tnaA, Ptac-fmoMa

Selection marker:

Ampicillin



Modifications
Gene Organism Modification
tnaA
Escherichia coli
Overexpression
fmo
Methylophaga aminisulfidivorans
Overexpression



Strains harboring plasmid pTrcTF1
  • Escherichia coli W3110/pTrcTF1
  • Escherichia coli IND2/pTrcTF1
  • Escherichia coli IND2/pTrcTF1/pTac-G
  • Escherichia coli IND2/pTrcTF1/pTac-GE1
  • Escherichia coli IND2/pTrcTF1/pTac-GE2
  • Escherichia coli IND2/pTrcTF1/pTac-GEL
  • Escherichia coli IND4/pTrcTF1/pTac-GEL
  • Escherichia coli IND5/pTrcTF1/pTac-GEL

References
  • Jikun Du, Gongsoo Yang, Zi Wei Luo, Sang Yup Lee (2018). Metabolic engineering of Escherichia coli for the production of indirubin from glucose. Journal of Biotechnology.

Reference

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