Escherichia coli IND4/pTrcTF1/pTac-GEL
Ancestors
Genotype with respect to wild type
F+ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | ΔtrpR | ΔpykF ΔpykA | Plasmid pTacGEL added | Plasmid pTrcTF1 addedBars (|) indicate differences between strains.
Plasmids
Modifications
| Origin | Gene | Organism | Modification |
|---|---|---|---|
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Silencing
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Silencing
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Silencing
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Overexpression
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Overexpression
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Feedback insensitive
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Feedback insensitive
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Overexpression
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Production
| Metabolites | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
|---|---|---|---|---|---|---|---|
| indigo | Titer | 104.0 mg/L | Flask | [12] | |||
| indigo | Titer | 0.629 g/L | 86 h | Fed-Batch | [12] | ||
| Indirubin | Titer | 0.033 g/L | 86 h | Fed-Batch | [12] |
* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.