Escherichia coli IND5/pTrcTF1/pTac-GEL
Ancestors
Genotype with respect to wild type
F+ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | ΔtrpR | ΔpykF ΔpykA | PtktA:Ptrc | Plasmid pTacGEL added | Plasmid pTrcTF1 addedBars (|) indicate differences between strains.
Plasmids
Modifications
| Origin | Gene | Organism | Modification |
|---|---|---|---|
|
Silencing
|
|||
|
Silencing
|
|||
|
Silencing
|
|||
|
Overexpression
|
|||
|
Overexpression
|
|||
|
Overexpression
|
|||
|
Feedback insensitive
|
|||
|
Feedback insensitive
|
|||
|
Overexpression
|
Production
| Metabolites | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
|---|---|---|---|---|---|---|---|
| indigo | Titer | 108.0 mg/L | 72 h | Flask | [12] | ||
| Indirubin | Titer | 0.056 g/L | 72 h | Fed-Batch | [12] | ||
| indigo | Titer | 0.64 g/L | 72 h | Fed-Batch | [12] |
* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.