pTyr-a

Ancestors

All plasmids

pTyr-a

Main features

P_tac, ColE1 ori, aroK, tyrC, aroG^A146N, tyrA^A354V,M53I, anti-csrA synthetic sRNA expression regulated by araC and temperature-sensitive cI^ts2

Selection marker:

Ampicillin

Modifications

Gene	Organism	Modification
*aroK*	Escherichia coli	Overexpression
*tyrC*	Zymomonas mobilis	Overexpression
*aroG*	Escherichia coli	Overexpression
*tyrA*	Escherichia coli	Overexpression
*csrA*	Escherichia coli	Attenuation

References

Jikun Du, Gongsoo Yang, Zi Wei Luo, Sang Yup Lee (2018). Metabolic engineering of Escherichia coli for the production of indirubin from glucose. Journal of Biotechnology.