pTac-GETD

Ancestors

All plasmids

pTac15K

pTac-G

pTac-GE1

pTacGED

pTac-GETD

Main features

P_tac, P_tac-trpE^S40F, aroG^A146N, P_tac-aroD, P_tac-tktA

Selection marker:

Kanamycin

Modifications

Gene	Organism	Modification
*trpE*	Escherichia coli	Feedback insensitive
*aroG*	Escherichia coli	Feedback insensitive
*aroD*	Escherichia coli	Overexpression
*tktA*	Escherichia coli	Overexpression

References

Jikun Du, Gongsoo Yang, Zi Wei Luo, Sang Yup Lee (2018). Metabolic engineering of Escherichia coli for the production of indirubin from glucose. Journal of Biotechnology.