pTac-GE2

Ancestors

All plasmids

pTac15K

pTac-G

pTac-GE2

Main features

P_tac, trpE^S40F, aroG^A146N

Selection marker:

Kanamycin

Modifications

Gene	Organism	Modification
*trpE*	Escherichia coli	Feedback insensitive
*aroG*	Escherichia coli	Feedback insensitive

References

Jikun Du, Gongsoo Yang, Zi Wei Luo, Sang Yup Lee (2018). Metabolic engineering of Escherichia coli for the production of indirubin from glucose. Journal of Biotechnology.