Escherichia coli FB-04/pSV03

Ancestors

Genotype with respect to parental

aroFfbr trpEfbrD


Genotype with respect to wild type
F+ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | ΔtrpR | ΔtnaA | ΔpheA | ΔtrpR::FRT ΔtnaA::FRT ΔpheA::FRT ΔtyrA::FRT | Plasmid pSV03 added | aroFfbr trpEfbrD

Bars (|) indicate differences between strains.


Plasmids

  • pSV03 (p15A ori, aroFfbr trpEfbrD)


Production
Metabolites Heterologous Production type Production Biomass Carbon source Time Scale Ref.
L-tryptophan Titer 13.3 g/L 41.2 g/L Glucose Fed-Batch [ 147 ]
L-tryptophan Substrate yield 0.1 g/g of sustrate 41.2 g/L Glucose Fed-Batch [ 147 ]
L-tryptophan Titer 1.1 g/L 7.0 OD600 Glucose 48 h Flask [ 146 ]
L-tryptophan Substrate yield 0.02 g/g of sustrate 7.0 OD600 Glucose 48 h Flask [ 146 ]
L-tryptophan Volumetric productivity 0.02 g/L/h 7.0 OD600 Glucose 48 h Flask [ 146 ]
acetate Titer 12.9 g/L 7.0 OD600 Glucose 48 h Flask [ 146 ]
L-tryptophan Titer 38.1 g/L 73.6 OD600 Glucose 54 h Fed-Batch [ 146 ]
L-tryptophan Substrate yield 0.11 g/g of sustrate 73.6 OD600 Glucose 54 h Fed-Batch [ 146 ]
L-tryptophan Volumetric productivity 0.7 g/L/h 73.6 OD600 Glucose 54 h Fed-Batch [ 146 ]
acetate Titer 4.3 g/L 73.6 OD600 Glucose 54 h Fed-Batch [ 146 ]

* Inferred from plots using RetroPlot.


References