Escherichia coli BWT03

Ancestors

All strains

Escherichia coli B

Escherichia coli BL21

Escherichia coli BL21(DE3)

Escherichia coli BWT01

Escherichia coli BWT03

Genotype with respect to wild type

F⁻ dcm gal ompT hsdS(r_B^- m_B^-) | F⁻ ompT hsdS_B (r_B⁻m_B⁻) gal dcm λ(DE3) | Plasmid pCDFDuet-AtCCR-Pc4CL-AtPAL added | Plasmid pET28a-UGT74R1 added

Bars (|) indicate differences between strains.

Plasmids

pCDFDuet-AtCCR-Pc4CL-AtPAL (CloDF13 ori, lacI, AtCCR, Pc4CL, AtPAL)

pET28a-UGT74R1 (pBR322 ori, f1 ori, P_T7-UGT74R1)

Modifications

Origin	Gene	Organism	Modification
pCDFDuet-AtCCR-Pc4CL-AtPAL	CCR1	Arabidopsis thaliana	Overexpression
pCDFDuet-AtCCR-Pc4CL-AtPAL	4CL	Petroselinum crispum	Overexpression
pCDFDuet-AtCCR-Pc4CL-AtPAL	PAL	Arabidopsis thaliana	Overexpression
pET28a-UGT74R1	UGT74R1	Rhodiola sachalinensis	Overexpression

References

Zhou, Wei; Bi, Huiping; Zhuang, Yibin; He, Qinglin; Yin, Hua; Liu, Tao & Ma, Yanhe. Production of Cinnamyl Alcohol Glucoside from Glucose in Escherichia coli. Journal of Agricultural and Food Chemistry. 2017, 65(10), 2129-2135.