Zhou, Wei; Bi, Huiping; Zhuang, Yibin; He, Qinglin; Yin, Hua; Liu, Tao & Ma, Yanhe. Production of Cinnamyl Alcohol Glucoside from Glucose in Escherichia coli. Journal of Agricultural and Food Chemistry. 2017, 65(10), 2129-2135.
Strains
- Escherichia coli BPHE
- Escherichia coli BWT01
- Escherichia coli BWT02
- Escherichia coli BWT03
- Escherichia coli BWT04
- Escherichia coli BWT05
- Escherichia coli BPHE01
- Escherichia coli BPHE02
- Escherichia coli BL21(DE3)
Plasmids
- pCDFDuet-1
- pET-28a(+)
- pET28a-AtPAL
- pCDFDuetAtCCR-Pc4CL
- pET28a-UGT72B14
- pET28a-UGT74R1
- pET28a-UGT73B6
- pET28a-UGT73C5
- pCDFDuet-AtCCR-Pc4CL-AtPAL
Metabolite production
-
cinnamyl alcohol
-
Titer
- 197.8 mg/L (Escherichia coli BWT01 in Flask)
- 118.4 mg/L (Escherichia coli BWT05 in Flask)
- 151.5 mg/L (Escherichia coli BWT04 in Flask)
- 285.3 mg/L (Escherichia coli BPHE01 in Flask)
-
rosin
-
Titer
- 57.3 mg/L (Escherichia coli BWT04 in Flask)
- 168.9 mg/L (Escherichia coli BWT05 in Flask)
- 258.5 mg/L (Escherichia coli BPHE02 in Flask)