Escherichia coli ΔpykF

Ancestors

Derived strains

Genotype with respect to parental

ΔpykF::FRT


Genotype with respect to wild type
F+ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | ΔtrpR | ΔtnaA | ΔpheA | ΔtrpR::FRT ΔtnaA::FRT ΔpheA::FRT ΔtyrA::FRT | Plasmid pSV03 added | Δpta::pta1 | ΔpykF::FRT

Bars (|) indicate differences between strains.


Plasmids

  • pSV03 (p15A ori, aroFfbr trpEfbrD)


Modifications
Gene Organism Modification
Silencing

Production
Metabolites Heterologous Production type Production Biomass Carbon source Time Scale Ref.
L-tryptophan Titer 2.43 g/L 9.0 OD600 Glucose 48 h Flask [ 175 ]
L-tryptophan Substrate yield 0.065 g/g of sustrate 9.0 OD600 Glucose 48 h Flask [ 175 ]
acetate Titer 6.79 g/L 9.0 OD600 Glucose 48 h Flask [ 175 ]
L-tryptophan Titer 45.5 g/L 72.2 OD600 Glucose Fed-Batch [ 175 ]
L-tryptophan Substrate yield 0.14 g/g of sustrate 72.2 OD600 Glucose Fed-Batch [ 175 ]
acetate Titer 1.9 g/L 72.2 OD600 Glucose Fed-Batch [ 175 ]

* Inferred from plots using RetroPlot.


References