Escherichia coli ΔpykF/pykA
Ancestors
Genotype with respect to parental
ΔpykF::FRT ΔpykA::FRT
Genotype with respect to wild type
F+ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | ΔtrpR | ΔtnaA | ΔpheA | ΔtrpR::FRT ΔtnaA::FRT ΔpheA::FRT ΔtyrA::FRT | Plasmid pSV03 added | Δpta::pta1 | ΔpykF::FRT ΔpykA::FRTBars (|) indicate differences between strains.
Plasmids
-
pSV03 (p15A ori, aroFfbr trpEfbrD)
Modifications
| Gene | Organism | Modification |
|---|---|---|
|
Silencing
|
||
|
Silencing
|
Production
| Metabolites | Heterologous | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
|---|---|---|---|---|---|---|---|---|
| L-tryptophan | Titer | 1.37 g/L | 5.8 OD600 | Glucose | 48 h | Batch | [ 175 ] | |
| L-tryptophan | Substrate yield | 0.051 g/g of sustrate | 5.8 OD600 | Glucose | 48 h | Batch | [ 175 ] | |
| acetate | Titer | 4.25 g/L | 5.8 OD600 | Glucose | 48 h | Flask | [ 175 ] |
* Inferred from plots using RetroPlot.