Escherichia coli T8

Ancestors

Derived strains

Genotype with respect to parental

ΔlacI, tyrR::Ptrc-aroGS180F, trpR::Ptrc-serAH344A,N364A, trpE::Ptrc-trpES40F, ΔpykF, tktA::Ptrc-tktA, ΔptsH, yjiV::PJ23105-UTRWP1-galP, ylbE::PJ23114-UTRMK3-glk, PrpsT p1-ppc, Prrnc p1-gltA

Genotype with respect to wild type

F⁺ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | ΔlacI, tyrR::Ptrc-aroGS180F, trpR::Ptrc-serAH344A,N364A, trpE::Ptrc-trpES40F, ΔpykF, tktA::Ptrc-tktA, ΔptsH, yjiV::PJ23105-UTRWP1-galP, ylbE::PJ23114-UTRMK3-glk, PrpsT p1-ppc, Prrnc p1-gltA

Bars (|) indicate differences between strains.

Modifications

Gene	Organism	Modification
lacI	Escherichia coli	Silencing
tyrR	Escherichia coli	Silencing
aroG	Escherichia coli	Feedback insensitive
trpR	Escherichia coli	Silencing
serA	Escherichia coli	Feedback insensitive
trpE	Escherichia coli	Feedback insensitive
pykF	Escherichia coli	Silencing
tktA	Escherichia coli	Overexpression
ptsH	Escherichia coli	Silencing
galP	Escherichia coli	Overexpression
glk	Escherichia coli	Overexpression
ppc	Escherichia coli	Overexpression
gltA	Escherichia coli	Overexpression

Production

Metabolites	Heterologous	Production type	Production	Biomass	Carbon source	Time	Scale	Ref.
L-tryptophan		Titer	9.7 g/L		Glucose		Flask	[ 183 ]
L-tryptophan		Substrate yield	0.129 g/g of sustrate		Glucose		Flask	[ 183 ]

* Inferred from plots using RetroPlot.

References

Liu S, Wang BB, Xu JZ, Zhang WG. Engineering of Shikimate Pathway and Terminal Branch for Efficient Production of L-Tryptophan in Escherichia coli. Int J Mol Sci. 2023 Jul 24;24(14):11866.