galP

Escherichia coli

Sequence

                atgCCTGACGCTAAAAAACAGGGGCGGTCAAACAAGGCAATGACGTTTTTCGTCTGCTTCCTTGCCGCTCTGGCGGGATTACTCTTTGGCCTGGATATCGGTGTAATTGCTGGCGCACTGCCGTTTATTGCAGATGAATTCCAGATTACTTCGCACACGCAAGAATGGGTCGTAAGCTCCATGATGTTCGGTGCGGCAGTCGGTGCGGTGGGCAGCGGCTGGCTCTCCTTTAAACTCGGGCGCAAAAAGAGCCTGATGATCGGCGCAATTTTGTTTGTTGCCGGTTCGCTGTTCTCTGCGGCTGCGCCAAACGTTGAAGTACTGATTCTTTCCCGCGTTCTACTGGGGCTGGCGGTGGGTGTGGCCTCTTATACCGCACCGCTGTACCTCTCTGAAATTGCGCCGGAAAAAATTCGTGGCAGTATGATCTCGATGTATCAGTTGATGATCACTATCGGGATCCTCGGTGCTTATCTTTCTGATACCGCCTTCAGCTACACCGGTGCATGGCGCTGGATGCTGGGTGTGATTATCATCCCGGCAATTTTGCTGCTGATTGGTGTCTTCTTCCTGCCAGACAGCCCACGTTGGTTTGCCGCCAAACGCCGTTTTGTTGATGCCGAACGCGTGCTGCTACGCCTGCGTGACACCAGCGCGGAAGCGAAACGCGAACTGGATGAAATCCGTGAAAGTTTGCAGGTTAAACAGAGTGGCTGGGCGCTGTTTAAAGAGAACAGCAACTTCCGCCGCGCGGTGTTCCTTGGCGTACTGTTGCAGGTAATGCAGCAATTCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGTGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGTACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCACCTGCTCCACTGCCACCAACTGGATTGCCAACATGATCGTTGGCGCAACGTTCCTGACCATGCTCAACACGCTGGGTAACGCCAACACCTTCTGGGTGTATGCGGCTCTGAACGTACTGTTTATCCTGCTGACATTGTGGCTGGTACCGGAAACCAAACACGTTTCGCTGGAACATATTGAACGTAATCTGATGAAAGGTCGTAAACTGCGCGAAATAGGCGCTCACGATtaa

The galP gene encodes galactose permease, which functions as a non-phosphotransferase system (non-PTS) active transporter responsible for the translocation of sugars across the cell membrane. It imports glucose into the cytoplasm, where it is phosphorylated by glucokinase using ATP as the phosphate donor, thereby bypassing the phosphoenolpyruvate (PEP)-dependent phosphotransferase system [343, 363]. Role in metabolic engineering: Deletion of the native glucose phosphotransferase system (PTS) is a common strategy to increase the intracellular pool of PEP for downstream biosynthetic pathways, but it typically impairs glucose consumption and growth [363]. Overexpression of the heterologous or native galP gene, frequently in combination with a glucokinase gene (glk), is implemented to completely restore sugar consumption and glycolytic flux without depleting the PEP pool [343, 361, 363]. This non-PTS transport approach preserves the precursor PEP to redirect carbon flux toward the overproduction of target compounds, particularly aromatic derivatives and L-aspartate-derived family amino acids [343, 363].

`Gene size:`
`Protein size:`
`Reactions`	R00800
`Compounds affected`	L-tyrosine , L-dopa , fumarate , 2-phenylethylamine , L-tryptophan , L-homoserine and L-phenylalanine

Databases

`EraGene:`	2197515
`UniProt:`	P0AEP1
`KEGG:`	eco:b2943