Escherichia coli T8K

Ancestors

All strains

Escherichia coli K12

Escherichia coli W1485

Escherichia coli W2637

Escherichia coli W3110

Escherichia coli T8

Escherichia coli T8K

Genotype with respect to parental

P_trc-aroK

Genotype with respect to wild type

F⁺ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | ΔlacI, tyrR::Ptrc-aroGS180F, trpR::Ptrc-serAH344A,N364A, trpE::Ptrc-trpES40F, ΔpykF, tktA::Ptrc-tktA, ΔptsH, yjiV::PJ23105-UTRWP1-galP, ylbE::PJ23114-UTRMK3-glk, PrpsT p1-ppc, Prrnc p1-gltA | Plasmid pSB4K5-aroK added | P_trc-aroK

Bars (|) indicate differences between strains.

Plasmids

pSB4K5-aroK (P_trc-aroK)

Production

Metabolites	Heterologous	Production type	Production	Biomass	Carbon source	Time	Scale	Ref.
L-tryptophan		Titer	10.16 g/L		Glucose		Flask	[ 183 ]
L-tryptophan		Substrate yield	0.131 g/g of sustrate		Glucose		Flask	[ 183 ]

* Inferred from plots using RetroPlot.

References

Liu S, Wang BB, Xu JZ, Zhang WG. Engineering of Shikimate Pathway and Terminal Branch for Efficient Production of L-Tryptophan in Escherichia coli. Int J Mol Sci. 2023 Jul 24;24(14):11866.