Escherichia coli JCL230

Ancestors

All strains

Escherichia coli K12

Escherichia coli BW25113

Escherichia coli JCL16

Escherichia coli JCL88

Escherichia coli JCL230

Genotype with respect to wild type

F⁺ (λ) | IacI^q rrnB_T14 ∆lacZ_WJ16 hsdR514 ∆araBAD_AH33 ∆rhaBAD_LD78 | F' [traD36, proAB⁺, lacI^q ZDM15] | ΔadhE, ΔldhA, ΔfrdBC, Δfnr, Δpta | Plasmid pJCL63 added | Plasmid pJCL17 added

Bars (|) indicate differences between strains.

Plasmids

pJCL17 (P_LlacO₁::atoB(EC)-adhE2(CA))

pJCL63 (P_LlacO₁::crt-bcd(ME)-ccr(SC)-hbd(CA))

Modifications

Origin	Gene	Organism	Modification
Escherichia coli JCL88	adhE	Escherichia coli	Silencing
Escherichia coli JCL88	ldhA	Escherichia coli	Silencing
Escherichia coli JCL88	frdB	Escherichia coli	Silencing
Escherichia coli JCL88	frdC	Escherichia coli	Silencing
Escherichia coli JCL88	fnr	Escherichia coli	Silencing
Escherichia coli JCL88	pta	Escherichia coli	Silencing
pJCL17	atoB	Escherichia coli	Overexpression
pJCL17	adhE	Clostridium acetobutylicum	Overexpression
pJCL63	hbd	Clostridium acetobutylicum	Overexpression
pJCL63	ccr	Streptomyces coelicolor	Overexpression
pJCL63	crt	Clostridium acetobutylicum	Overexpression

Production

Metabolites	Production type	Production	Biomass	Carbon source	Time	Scale	Ref.
butan-1-ol	Titer	2.0 mg/L ^*		Glucose		Flask	[90]

* Inferred from plots using RetroPlot.

Heterologous.

Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References

Atsumi, Shota; Cann, Anthony F.; Connor, Michael R.; Shen, Claire R.; Smith, Kevin M.; Brynildsen, Mark P.; Chou, Katherine J.Y.; Hanai, Taizo & Liao, James C. (2008). Metabolic engineering of Escherichia coli for 1-butanol production. Metabolic Engineering, 10(6), 305-311.