Escherichia coli TS-7

Ancestors

All strains

Escherichia coli K12

Escherichia coli W1485

Escherichia coli W2637

Escherichia coli W3110

Escherichia coli KW

Escherichia coli KW001

Escherichia coli TS-1

Escherichia coli TS-5

Escherichia coli TS-6

Escherichia coli TS-7

Derived strains

Escherichia coli TS-8

Genotype with respect to parental

serA^mut

Genotype with respect to wild type

F⁺ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | Plasmid PH5a1 added | ΔglnA::BSglnA | ΔackA::J23119-icd ΔpoxB::J23119-gdhA | ΔatpI::pTrc::prs | serA^mut

Bars (|) indicate differences between strains.

Plasmids

PH5a1 (aroG^fbr trpEDCBA)

Modifications

Gene	Organism	Modification
serA	Escherichia coli	Feedback insensitive

Production

Metabolites	Heterologous	Production type	Production	Biomass	Carbon source	Time	Scale	Ref.
2-oxoglutarate		Titer	1.241 g/L		Glucose	48 h	Flask	[ 136 ]
acetate		Titer	0.276 g/L		Glucose	48 h	Flask	[ 136 ]
L-tryptophan		Titer	1.04 g/L		Glucose	48 h	Flask	[ 136 ]

* Inferred from plots using RetroPlot.

References

Zhu Li, Dongqin Ding, Huiying Wang, Linxia Liu, Huan Fang, Tao Chen & Dawei Zhang (2020). Engineering Escherichia coli to improve tryptophan production via genetic manipulation of precursor and cofactor pathways. Synthetic and Systems Biotechnology.