Escherichia coli CWF812

Ancestors

Genotype with respect to parental

PgalP::Ptrc


Genotype with respect to wild type
F+ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | ΔiclR | ΔfumC | ΔfumA | ΔfumB | Plasmid pTac15kppc added | ΔarcA | ΔptsG | ΔaspA | ΔlacI | PgalP::Ptrc

Bars (|) indicate differences between strains.


Plasmids

Modifications
Gene Organism Modification
Overexpression

Production
Metabolites Heterologous Production type Production Biomass Carbon source Time Scale Ref.
fumarate Titer 7.85 g/L Glucose 20 h Batch [ 210 ]
fumarate Substrate yield 0.394 g/g of sustrate Glucose 20 h Batch [ 210 ]
fumarate Volumetric productivity 0.393 g/L/h Glucose 20 h Batch [ 210 ]
fumarate Specific production rate 0.238 g/g DCW/h Glucose 20 h Batch [ 210 ]
acetate Titer 2.0 g/L Glucose 20 h Batch [ 210 ]
acetate Substrate yield 0.1 g/g of sustrate Glucose 20 h Batch [ 210 ]
fumarate Titer 28.2 g/L Glucose 63 h Fed-Batch [ 210 ]
fumarate Substrate yield 0.389 g/g of sustrate Glucose 63 h Fed-Batch [ 210 ]
fumarate Volumetric productivity 0.448 g/L/h Glucose 63 h Fed-Batch [ 210 ]
acetate Titer 5.24 g/L Glucose 63 h Fed-Batch [ 210 ]
acetate Substrate yield 0.072 g/g of sustrate Glucose 63 h Fed-Batch [ 210 ]

* Inferred from plots using RetroPlot.


References