Escherichia coli 050T4-1/pCTSDT

Ancestors

All strains

Escherichia coli B0016

Escherichia coli B0016-050

Escherichia coli 050T

Escherichia coli 050T3

Escherichia coli 050T4-1

Escherichia coli 050T4-1/pCTSDT

Genotype with respect to wild type

Δack-pta ΔpflB ΔadhE ΔfrdA ΔldhA | ilvE::T7RNAP | ΔleuA | P_pntA::P_TM1 | Plasmid pCTSDT added

Bars (|) indicate differences between strains.

Plasmids

pCTSDT (P_T7-ilvC_Ec-T_T7-P_T7-alsS_Bs-P_T7-ilvD_Ec-T_T7)

Production

Metabolites	Heterologous	Production type	Production	Biomass	Carbon source	Time	Scale	Ref.
3-methyl-2-oxobutanoate		Titer	7.56 g/L ^*	9.1 OD₆₀₀^*	Glucose		Flask	[ 221 ]

* Inferred from plots using RetroPlot.

References

Li Zhou, Ying Zhu, Zhongzhe Yuan, Guangqing Liu, Zijin Sun, Shiyu Du, He Liu, Yating Li, Haili Liu, and Zhemin Zhou (2022). Evaluation of Metabolic Engineering Strategies on 2-Ketoisovalerate Production by Escherichia coli. Applied and Environmental Microbiology. 88(17): e00976-22.