Escherichia coli XBR11

Ancestors

All strains

Escherichia coli K12

Escherichia coli BW25113

Escherichia coli BW25113/F'

Escherichia coli XY01

Escherichia coli XY02

Escherichia coli XY04

Escherichia coli XY11

Escherichia coli XBR11

Genotype with respect to wild type

F⁺ (λ) | IacI^q rrnB_T14 ∆lacZ_WJ16 hsdR514 ∆araBAD_AH33 ∆rhaBAD_LD78 | F׳ proAB lacI^qZΔM15 Tn10 (Tet^r) | ΔompT::P_tac-ppc_Cg | ΔpykA ΔpykF | Δmdh | ΔaspA | Plasmid pLB1a-CR added | Plasmid pXB1k-BD added

Bars (|) indicate differences between strains.

Plasmids

pXB1k-BD (P_araBAD-panD_Bs)

pLB1a-CR (P_araBAD-aspC_Ec rocG_Bs)

Production

Metabolites	Heterologous	Production type	Production	Biomass	Carbon source	Time	Scale	Ref.
β-alanine		Titer	39.66 mM ^*		Glucose		Flask	[ 223 ]
acetate		Titer	26.36 mM ^*		Glucose		Flask	[ 223 ]
(R)-lactate		Titer	6.74 mM ^*		Glucose		Flask	[ 223 ]
formate		Titer	9.06 mM ^*		Glucose		Flask	[ 223 ]
β-alanine		Substrate yield	0.79 mol/mol of substrate		Glucose		Flask	[ 223 ]
β-alanine		Titer	48.26 mM ^*		Glucose		Flask	[ 223 ]
acetate		Titer	25.33 mM ^*		Glucose		Flask	[ 223 ]
(R)-lactate		Titer	11.43 mM ^*		Glucose		Flask	[ 223 ]
formate		Titer	5.53 mM ^*		Glucose		Flask	[ 223 ]
succinate		Titer	3.44 mM ^*		Glucose		Flask	[ 223 ]
succinate		Titer	2.0 mM ^*		Glucose		Flask	[ 223 ]

* Inferred from plots using RetroPlot.

References

Xiaoyu Piao, Lei Wang, Baixue Lin, Hao Chen, Weifeng Liu & Yong Tao (2019). Metabolic engineering of Escherichia coli for production of L-aspartate and its derivative β-alanine with high stoichiometric yield. Metabolic Engineering.