Escherichia coli FB-04(ΔackA)

Ancestors

Genotype with respect to parental

ΔackA::kan-sacB


Genotype with respect to wild type
F+ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | ΔtrpR | ΔtnaA | ΔpheA | ΔtrpR::FRT ΔtnaA::FRT ΔpheA::FRT ΔtyrA::FRT | Plasmid pSV03 added | ΔackA::kan-sacB

Bars (|) indicate differences between strains.


Plasmids

  • pSV03 (p15A ori, aroFfbr trpEfbrD)


Modifications
Gene Organism Modification
Silencing
Overexpression

Production
Metabolites Heterologous Production type Production Biomass Carbon source Time Scale Ref.
L-tryptophan Titer 1.3 g/L 7.7 OD600 Glucose 48 h Flask [ 146 ]
L-tryptophan Substrate yield 0.032 g/g of sustrate 7.7 OD600 Glucose 48 h Flask [ 146 ]
L-tryptophan Volumetric productivity 0.74 g/L/h 7.7 OD600 Glucose 48 h Flask [ 146 ]
acetate Titer 4.9 g/L 7.7 OD600 Glucose 48 h Flask [ 146 ]
L-tryptophan Titer 40.2 g/L 76.8 OD600 Glucose 54 h Fed-Batch [ 146 ]
L-tryptophan Substrate yield 0.12 g/g of sustrate 76.8 OD600 Glucose 54 h Fed-Batch [ 146 ]
L-tryptophan Volumetric productivity 0.74 g/L/h 76.8 OD600 Glucose 54 h Fed-Batch [ 146 ]
acetate Titer 1.5 g/L 76.8 OD600 Glucose 54 h Fed-Batch [ 146 ]

* Inferred from plots using RetroPlot.


References