Escherichia coli FB-04(pta1)

Ancestors
Derived strains

Genotype with respect to parental

Δpta::pta1


Genotype with respect to wild type
F+ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | ΔtrpR | ΔtnaA | ΔpheA | ΔtrpR::FRT ΔtnaA::FRT ΔpheA::FRT ΔtyrA::FRT | Plasmid pSV03 added | Δpta::pta1

Bars (|) indicate differences between strains.


Plasmids
  • pSV03 (p15A ori, aroFfbr trpEfbrD)


Modifications
Gene Organism Modification
pta
Attenuation

Production
Metabolites Heterologous Production type Production Biomass Carbon source Time Scale Ref.
L-tryptophan Titer 2.09 g/L 7.5 OD600 Glucose 48 h Flask [ 146 ]
L-tryptophan Substrate yield 0.049 g/g of sustrate 7.5 OD600 Glucose 48 h Flask [ 146 ]
L-tryptophan Volumetric productivity 0.044 g/L/h 7.5 OD600 Glucose 48 h Flask [ 146 ]
acetate Titer 8.4 g/L 7.5 OD600 Glucose 48 h Flask [ 146 ]
acetate Titer 2.1 g/L 82.8 OD600 Glucose 54 h Fed-Batch [ 146 ]
L-tryptophan Titer 44.0 g/L 82.8 OD600 Glucose 54 h Fed-Batch [ 146 ]
L-tryptophan Substrate yield 0.13 g/g of sustrate 82.8 OD600 Glucose 54 h Fed-Batch [ 146 ]
L-tryptophan Volumetric productivity 0.82 g/L/h 82.8 OD600 Glucose 54 h Fed-Batch [ 146 ]

* Inferred from plots using RetroPlot.


References