Escherichia coli W-14/pM1/pR1

Ancestors

All strains

Escherichia coli K12

Escherichia coli W1485

Escherichia coli W2637

Escherichia coli W3110

Escherichia coli W-H4

Escherichia coli W-H7

Escherichia coli W-H8

Escherichia coli W-H9

Escherichia coli W-H10

Escherichia coli W-H14

Escherichia coli W-14/pM1/pR1

Genotype with respect to wild type

F⁺ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | ΔlysA ΔthrB ΔmetA ΔlacI | ΔldhA ΔadhE ΔpflB | ΔptsG | ΔP_galP::P_lac | ΔP_ppc::P_trc | ΔP_aspA::P_trc | Plasmid pR1 added | Plasmid pM1 added

Bars (|) indicate differences between strains.

Plasmids

pM1 (P_grac/RBS6-metL)

pR1 (P_rhtA-rhtA)

Production

Metabolites	Heterologous	Production type	Production	Biomass	Carbon source	Time	Scale	Ref.
L-homoserine		Titer	63.7 mM ^*	15.84 OD₆₀₀^*	Glucose	36 h ^*	Flask	[ 238 ]

* Inferred from plots using RetroPlot.

References

Vo, Toan Minh & Park, Sunghoon. Metabolic engineering of Escherichia coli W3110 for efficient production of homoserine from glucose. Metabolic Engineering. 2022, 73, 104-113.