Kwang Ho Lee, Jin Hwan Park, Tae Yong Kim, Hyun Uk Kim & Sang Yup Lee (2007). Systems metabolic engineering of Escherichia coli for L‐threonine production. Molecular Systems Biology.

Strains

• Escherichia coli TH01
• Escherichia coli TH02
• Escherichia coli TH03
• Escherichia coli TH04
• Escherichia coli TH05
• Escherichia coli TH06
• Escherichia coli TH07
• Escherichia coli TH09C
• Escherichia coli TH11C
• Escherichia coli TH19C
• Escherichia coli TH20C
• Escherichia coli TH27C
• Escherichia coli TH28C
• Escherichia coli TH07(pBRThrABC)
• Escherichia coli TH07 (pBRThrABC, pACYC177)
• Escherichia coli TH07 (pBRThrABC, pACYCppc)
• Escherichia coli TH09C (pBRThrABC)
• Escherichia coli TH09C (pBRThrABC, pACYC177)
• Escherichia coli TH09C (pBRThrABC, pACYCaceBA)
• Escherichia coli TH11C (pBRThrABC, pACYC177)
• Escherichia coli TH19C (pBRThrABC)
• Escherichia coli TH20C (pBRThrABC)
• Escherichia coli TH27C (pBRThrABC)
• Escherichia coli TH27C (pBRThrABCR)
• Escherichia coli TH27C (pBRThrABCR2)
• Escherichia coli TH27C (pBRThrABCR3)
• Escherichia coli TH28C (pBRThrABCR3)
• Escherichia coli WL3110
• Escherichia coli W3110

Plasmids

• pBR322
• pKK223-3
• pACYCppc
• pACYCaceBA
• pBRThrABCR
• pBRThrABCR2
• pBRThrABCR3
• pUC19
• pACYC177
• pBRThrABC
• pACYC184

Metabolite production

• acetate
  Titer
  • 0.7 g/L (Escherichia coli TH07(pBRThrABC) in Batch)
  • 0.36 g/L (Escherichia coli TH09C (pBRThrABC) in Batch)
  • 2.16 g/L (Escherichia coli TH28C (pBRThrABCR3) in Fed-Batch)
  • 2.41 g/L (Escherichia coli TH28C (pBRThrABCR3) in Fed-Batch)
  • 0.62 g/L (Escherichia coli TH20C (pBRThrABC) in Batch)
  • 0.77 g/L (Escherichia coli TH27C (pBRThrABC) in Batch)
  • 0.8 g/L (Escherichia coli TH27C (pBRThrABCR) in Batch)
  • 0.59 g/L (Escherichia coli TH27C (pBRThrABCR2) in Batch)
  • 0.45 g/L (Escherichia coli TH27C (pBRThrABCR3) in Batch)
  • 0.64 g/L (Escherichia coli TH07 (pBRThrABC, pACYC177) in Batch)
  • 0.34 g/L (Escherichia coli TH07 (pBRThrABC, pACYCppc) in Batch)
  • 0.36 g/L (Escherichia coli TH09C (pBRThrABC, pACYC177) in Batch)
  • 0.05 g/L (Escherichia coli TH09C (pBRThrABC, pACYCaceBA) in Batch)
  • 1.91 g/L (Escherichia coli TH11C (pBRThrABC, pACYC177) in Batch)
  • 0.51 g/L (Escherichia coli TH19C (pBRThrABC) in Batch)
  • 0.24 g/L (Escherichia coli TH20C (pBRThrABC) in Batch)
  • 7.85 g/L (Escherichia coli TH27C (pBRThrABCR3) in Fed-Batch)
  • 2.35 g/L (Escherichia coli TH28C (pBRThrABCR3) in Fed-Batch)
• succinate
  Titer
  • 0.02 g/L (Escherichia coli TH07(pBRThrABC) in Batch)
  • 0.04 g/L (Escherichia coli TH09C (pBRThrABC) in Batch)
  • 0.01 g/L (Escherichia coli TH07 (pBRThrABC, pACYC177) in Batch)
  • 0.1 g/L (Escherichia coli TH07 (pBRThrABC, pACYCppc) in Batch)
  • 0.04 g/L (Escherichia coli TH09C (pBRThrABC, pACYC177) in Batch)
  • 0.15 g/L (Escherichia coli TH09C (pBRThrABC, pACYCaceBA) in Batch)
  • 0.0 g/L (Escherichia coli TH11C (pBRThrABC, pACYC177) in Batch)
  • 0.02 g/L (Escherichia coli TH19C (pBRThrABC) in Batch)
  • 0.03 g/L (Escherichia coli TH20C (pBRThrABC) in Batch)
• pyruvate
  Titer
  • 0.13 g/L (Escherichia coli TH07(pBRThrABC) in Batch)
  • 0.02 g/L (Escherichia coli TH09C (pBRThrABC) in Batch)
  • 0.12 g/L (Escherichia coli TH07 (pBRThrABC, pACYC177) in Batch)
  • 0.04 g/L (Escherichia coli TH07 (pBRThrABC, pACYCppc) in Batch)
  • 0.04 g/L (Escherichia coli TH09C (pBRThrABC, pACYC177) in Batch)
  • 0.02 g/L (Escherichia coli TH09C (pBRThrABC, pACYCaceBA) in Batch)
  • 2.21 g/L (Escherichia coli TH11C (pBRThrABC, pACYC177) in Batch)
  • 0.05 g/L (Escherichia coli TH19C (pBRThrABC) in Batch)
  • 0.03 g/L (Escherichia coli TH20C (pBRThrABC) in Batch)
• L-threonine
  Substrate yield
  • 0.294 g/g of sustrate (Escherichia coli TH28C (pBRThrABCR3) in Fed-Batch)
  • 0.273 g/g of sustrate (Escherichia coli TH28C (pBRThrABCR3) in Fed-Batch)
  • 0.268 g/g of sustrate (Escherichia coli TH28C (pBRThrABCR3) in Fed-Batch)
  • 0.246 g/g of sustrate (Escherichia coli TH27C (pBRThrABC) in Batch)
  • 0.37 g/g of sustrate (Escherichia coli TH27C (pBRThrABCR) in Batch)
  • 0.386 g/g of sustrate (Escherichia coli TH27C (pBRThrABCR2) in Batch)
  • 0.393 g/g of sustrate (Escherichia coli TH27C (pBRThrABCR3) in Batch)
  • 0.275 g/g of sustrate (Escherichia coli TH27C (pBRThrABCR3) in Fed-Batch)
  • 0.213 g/g of sustrate (Escherichia coli TH20C (pBRThrABC) in Batch)
  • 0.213 g/g of sustrate (Escherichia coli TH20C (pBRThrABC) in Batch)
  • 0.86 g/g of sustrate (Escherichia coli TH09C (pBRThrABC) in Batch)
  • 0.186 g/g of sustrate (Escherichia coli TH09C (pBRThrABC, pACYC177) in Batch)
  • 0.169 g/g of sustrate (Escherichia coli TH09C (pBRThrABC, pACYCaceBA) in Batch)
  • 0.028 g/g of sustrate (Escherichia coli TH11C (pBRThrABC, pACYC177) in Batch)
  • 0.183 g/g of sustrate (Escherichia coli TH19C (pBRThrABC) in Batch)
  • 0.202 g/g of sustrate (Escherichia coli TH07(pBRThrABC) in Flask)
  • 0.137 g/g of sustrate (Escherichia coli TH07(pBRThrABC) in Batch)
  • 0.14 g/g of sustrate (Escherichia coli TH07 (pBRThrABC, pACYC177) in Batch)
  • 0.118 g/g of sustrate (Escherichia coli TH07 (pBRThrABC, pACYCppc) in Batch)
  Titer
  • 82.4 g/L (Escherichia coli TH28C (pBRThrABCR3) in Fed-Batch)
  • 76.5 g/L (Escherichia coli TH28C (pBRThrABCR3) in Fed-Batch)
  • 75.1 g/L (Escherichia coli TH28C (pBRThrABCR3) in Fed-Batch)
  • 7.39 g/L (Escherichia coli TH27C (pBRThrABC) in Batch)
  • 11.1 g/L (Escherichia coli TH27C (pBRThrABCR) in Batch)
  • 11.59 g/L (Escherichia coli TH27C (pBRThrABCR2) in Batch)
  • 11.81 g/L (Escherichia coli TH27C (pBRThrABCR3) in Batch)
  • 77.1 g/L (Escherichia coli TH27C (pBRThrABCR3) in Fed-Batch)
  • 2.24 g/L (Escherichia coli TH20C (pBRThrABC) in Batch)
  • 6.39 g/L (Escherichia coli TH20C (pBRThrABC) in Batch)
  • 1.93 g/L (Escherichia coli TH09C (pBRThrABC) in Batch)
  • 1.92 g/L (Escherichia coli TH09C (pBRThrABC, pACYC177) in Batch)
  • 1.74 g/L (Escherichia coli TH09C (pBRThrABC, pACYCaceBA) in Batch)
  • 0.19 g/L (Escherichia coli TH11C (pBRThrABC, pACYC177) in Batch)
  • 1.89 g/L (Escherichia coli TH19C (pBRThrABC) in Batch)
  • 10.1 g/L (Escherichia coli TH07(pBRThrABC) in Flask)
  • 1.48 g/L (Escherichia coli TH07(pBRThrABC) in Batch)
  • 1.46 g/L (Escherichia coli TH07 (pBRThrABC, pACYC177) in Batch)
  • 1.21 g/L (Escherichia coli TH07 (pBRThrABC, pACYCppc) in Batch)