Xiaoyu Piao, Lei Wang, Baixue Lin, Hao Chen, Weifeng Liu & Yong Tao (2019). Metabolic engineering of Escherichia coli for production of L-aspartate and its derivative β-alanine with high stoichiometric yield. Metabolic Engineering.

Strains

Plasmids

Metabolite production

L-alanine
- 0.7 mM (Escherichia coli BW25113/F' in Flask)
- 2.7 mM (Escherichia coli XA00 in Flask)
- 4.02 mM (Escherichia coli XA01 in Flask)
- 5.15 mM (Escherichia coli XA02 in Flask)
- 1.34 mM (Escherichia coli XB00 in Flask)
- 1.38 mM (Escherichia coli XB01 in Flask)
- 1.42 mM (Escherichia coli XB02 in Flask)
- 0.12 mM (Escherichia coli XA04 in Flask)
- 1.47 mM (Escherichia coli XA08 in Flask)
- 0.11 mM (Escherichia coli XA09 in Flask)
- 0.22 mM (Escherichia coli XA11 in Flask)
- 0.16 mM (Escherichia coli XA12 in Flask)
acetate
- 26.36 mM (Escherichia coli XBR11 in Flask)
- 25.33 mM (Escherichia coli XBR11 in Flask)
- 6.57 mM (Escherichia coli XBR21 in Flask)
(R)-lactate
- 6.74 mM (Escherichia coli XBR11 in Flask)
- 11.43 mM (Escherichia coli XBR11 in Flask)
- 2.4 mM (Escherichia coli XBR21 in Flask)
β-alanine
- 65.44 mM (Escherichia coli XBR21 in Flask)
- 71.78 mM (Escherichia coli XBR22 in Flask)
- 48.26 mM (Escherichia coli XBR11 in Flask)
- 54.6 mM (Escherichia coli XBR21 in Flask)
- 63.13 mM (Escherichia coli XBR27 in Flask)
- 71.42 mM (Escherichia coli XAR22 in Flask)
- 368.0 mM (Escherichia coli XBR22 in Fed-Batch)
- 424.0 mM (Escherichia coli XBR41 in Fed-Batch)
- 0.0 mM (Escherichia coli BW25113/F' in Flask)
- 10.02 mM (Escherichia coli XB11 in Flask)
- 9.9 mM (Escherichia coli XB11 in Flask)
- 14.05 mM (Escherichia coli XD11-1 in Flask)
- 9.8 mM (Escherichia coli XD11-2 in Flask)
- 12.68 mM (Escherichia coli XD11-3 in Flask)
- 32.17 mM (Escherichia coli XBG11 in Flask)
- 39.66 mM (Escherichia coli XBR11 in Flask)
- 10.21 mM (Escherichia coli XB02 in Flask)
- 9.06 mM (Escherichia coli XB01 in Flask)
- 1.38 mM (Escherichia coli XB00 in Flask)
- 1.3 mol/mol of substrate (Escherichia coli XBR21 in Flask)
- 1.44 mol/mol of substrate (Escherichia coli XBR22 in Flask)
- 0.86 mol/mol of substrate (Escherichia coli XBR41 in Fed-Batch)
- 0.79 mol/mol of substrate (Escherichia coli XBR11 in Flask)
L-aspartate
- 50.12 mM (Escherichia coli XAR22 in Flask)
- 249.0 mM (Escherichia coli XAR31 in Fed-Batch)
- 6.1 mM (Escherichia coli XA11 in Flask)
- 6.91 mM (Escherichia coli XA11 in Flask)
- 21.04 mM (Escherichia coli XAG11 in Flask)
- 28.75 mM (Escherichia coli XAR11 in Flask)
- 0.0 mM (Escherichia coli XA04 in Flask)
- 5.5 mM (Escherichia coli XA12 in Flask)
- 0.0 mM (Escherichia coli XA09 in Flask)
- 0.11 mM (Escherichia coli XA08 in Flask)
- 0.1 mM (Escherichia coli XA02 in Flask)
- 0.0 g/L (Escherichia coli XA00 in Flask)
- 1.01 mol/mol of substrate (Escherichia coli XAR22 in Flask)
- 0.53 mol/mol of substrate (Escherichia coli XAR31 in Fed-Batch)
formate
- 5.53 mM (Escherichia coli XBR11 in Flask)
- 0.0 mM (Escherichia coli XBR21 in Flask)
- 9.06 mM (Escherichia coli XBR11 in Flask)
succinate
- 3.44 mM (Escherichia coli XBR11 in Flask)
- 4.49 mM (Escherichia coli XBR21 in Flask)
- 2.0 mM (Escherichia coli XBR11 in Flask)
- 2.04 mM (Escherichia coli XBR22 in Flask)
- 7.62 mM (Escherichia coli XBR27 in Flask)