Xinyu Zou, Laixian Guo, Lilong Huang, Miao Li, Sheng Zhang, Anren Yang, Yu Zhang, Luying Zhu, Hongxia Zhang, Juan Zhang & Zhibin Feng (2020). Pathway construction and metabolic engineering for fermentative production of β-alanine in Escherichia coli. Applied Microbiology & Biotechnology.
Strains
- Escherichia coli W3110
- Escherichia coli FZβA-1
- Escherichia coli FZβA-2
- Escherichia coli FZβA-3
- Escherichia coli FZβA-4
- Escherichia coli FZβA-5
- Escherichia coli FZβA-6
- Escherichia coli FZβA-7
- Escherichia coli FZβA-7-28am
- Escherichia coli FZβA-7-native
- Escherichia coli FZβA-8
- Escherichia coli FZβA-9
- Escherichia coli FZβA-10
- Escherichia coli FZβA-101
- Escherichia coli PpET28am-Pt7RBS-BtADC
- Escherichia coli PpET28am-PadcRBS-BtADC
- Escherichia coli PadcPt7RBS-BtADC
- Escherichia coli Padc-PadcRBS-BtADC
- Escherichia coli PldhA-Pt7RBS-BtADC
- Escherichia coli PldhA-PadcRBS-BtADC
Plasmids
Metabolite production
-
β-alanine
-
Titer
- 2.76 g/L (Escherichia coli FZβA-1 in Flask)
- 4.43 g/L (Escherichia coli FZβA-4 in Flask)
- 2.34 g/L (Escherichia coli PpET28am-Pt7RBS-BtADC in Flask)
- 1.8 g/L (Escherichia coli PpET28am-PadcRBS-BtADC in Flask)
- 1.41 g/L (Escherichia coli PadcPt7RBS-BtADC in Flask)
- 1.32 g/L (Escherichia coli Padc-PadcRBS-BtADC in Flask)
- 0.93 g/L (Escherichia coli PldhA-Pt7RBS-BtADC in Flask)
- 0.82 g/L (Escherichia coli PldhA-PadcRBS-BtADC in Flask)
- 3.39 g/L (Escherichia coli FZβA-2 in Flask)
- 5.82 g/L (Escherichia coli FZβA-5 in Flask)
- 5.12 g/L (Escherichia coli FZβA-6 in Flask)
- 8.71 g/L (Escherichia coli FZβA-6 in Flask)
- 9.22 g/L (Escherichia coli FZβA-7 in Flask)
- 9.22 g/L (Escherichia coli FZβA-7-28am in Flask)
- 12.32 g/L (Escherichia coli FZβA-8 in Flask)
- 18.21 g/L (Escherichia coli FZβA-9 in Flask)
- 24.51 g/L (Escherichia coli FZβA-10 in Flask) Volumetric productivity
- 0.89 g/L/h (Escherichia coli FZβA-10 in Fed-Batch)
-
L-glutamate
-
Titer
- 1.23 g/L (Escherichia coli FZβA-5 in Flask)
-
fumarate
-
Titer
- 1.22 g/L (Escherichia coli FZβA-7-28am in Flask)